Measurement of platelet aggregation functions using whole blood migration ratio in a microfluidic chip

Hong Seog Seo, Sung Hyuk Choi, Miran Han, Kyeong Ah Kim, Chy Hyun Cho, Seong Soo A An, Chae Seung Lim, Sehyun Shin

Research output: Contribution to journalArticle

Abstract

Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r=0.61, p <0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =-0.95, p <0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.

Original languageEnglish
Pages (from-to)151-163
Number of pages13
JournalClinical Hemorheology and Microcirculation
Volume62
Issue number2
DOIs
Publication statusPublished - 2016 Mar 18

Fingerprint

Microfluidics
Platelet Aggregation
Bleeding Time
Blood Platelets
Adenosine Diphosphate
Purinergic P2 Receptors
Hemostasis
Germany
Collagen
Outcome Assessment (Health Care)
Therapeutics

Keywords

  • ADP
  • Collagen
  • PFA-100
  • Platelet

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

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abstract = "Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r=0.61, p <0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =-0.95, p <0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.",
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AU - An, Seong Soo A

AU - Lim, Chae Seung

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