TY - JOUR
T1 - Measurement of platelet aggregation functions using whole blood migration ratio in a microfluidic chip
AU - Seo, Hong Seog
AU - Choi, Sung Hyuk
AU - Han, Miran
AU - Kim, Kyeong Ah
AU - Cho, Chi Hyun
AU - An, Seong Soo A.
AU - Lim, Chae Seung
AU - Shin, Sehyun
N1 - Funding Information:
This study was supported by a grant from the Korea Health technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI14C0670). This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C0670).
Publisher Copyright:
© 2016 - IOS Press and the authors. All rights reserved.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/3/18
Y1 - 2016/3/18
N2 - Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r=0.61, p < 0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =-0.95, p < 0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.
AB - Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r=0.61, p < 0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =-0.95, p < 0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.
KW - ADP
KW - Collagen
KW - PFA-100
KW - Platelet
UR - http://www.scopus.com/inward/record.url?scp=84961768996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961768996&partnerID=8YFLogxK
U2 - 10.3233/CH-151961
DO - 10.3233/CH-151961
M3 - Article
C2 - 26444593
AN - SCOPUS:84961768996
VL - 62
SP - 151
EP - 163
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
SN - 1386-0291
IS - 2
ER -