Measurement of the Nucleus Area and Nucleus/Cytoplasm and Mitochondria/Nucleus Ratios in Human Colon Tissues by Dual-Colour Two-Photon Microscopy Imaging

Chang Su Lim, Eun-Sun Kim, Ji Yeon Kim, Seung Taek Hong, Hoon-Jai Chun, Dong Eun Kang, Bong-Rae Cho

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Abstract

We developed two-photon (TP) probes for DNA (ABI-Nu), cytoplasm (Pyr-CT), and mitochondria (BF-MT). We found that ABI-Nu binds to AT in the minor groove, while ABI-Nu and BF-MT are effective for tracking in the cytoplasm and mitochondria, respectively. These probes showed very large effective two-photon action cross section values of 2230, 1555, and 790 Göppert-Mayer units (1GM=10-50 cm4s photon-1 molecule-1) at 740nm with emission maxima at 473, 561, and 560nm, respectively, in each organelle. Using these probes, we quantitatively estimated the mean nuclear area and the ratios of nuclei to cytoplasm and mitochondria to nuclei in human colon tissues by dual-colour two-photon microscopy imaging within 2 h after biopsy. The mean nuclear area and the nuclei to cytoplasm and mitochondria to cytoplasm ratios increased in the following order: normal colon mucosa

Original languageEnglish
Article number18521
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 17

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