Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection

P. Stoller, Beop-Min Kim, A. M. Rubenchik, K. M. Reiser, L. B. Da Silva

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

The measurement of the second order nonlinear susceptibility of collagen in various biological tissues has potential applications in the detection of structural changes which are related to different pathological conditions. We investigate second harmonic generation in rat-tail tendon, a highly organized collagen structure consisting of parallel fibers. Using an electro-optic modulator and a quarter-wave plate, we modulate the linear polarization of an ultra-short pulse laser beam that is used to measure second harmonic generation (SHG) in a confocal microscopy setup. Phase-sensitive detection of the generated signal, coupled with a simple model of the collagen protein structures, allows us to measure a parameter γ related to nonlinear susceptibility and to determine the relative orientation of the structures. Our preliminary results indicate that it may be possible to use this parameter to characterize the structure.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.F. Haglund, J. Neev, R.F. Wood
Pages11-16
Number of pages6
Volume4276
DOIs
Publication statusPublished - 2001
Externally publishedYes
EventCommercial and Biomedical Applications of Ultrashort Pulse Lasers; Laser Plasma Generation and Diagnostics - San Jose, CA, United States
Duration: 2001 Jan 232001 Jan 23

Other

OtherCommercial and Biomedical Applications of Ultrashort Pulse Lasers; Laser Plasma Generation and Diagnostics
CountryUnited States
CitySan Jose, CA
Period01/1/2301/1/23

Fingerprint

polarization modulation
collagens
Collagen
Modulation
Polarization
Harmonic generation
magnetic permeability
harmonic generations
Ultrafast lasers
tendons
Confocal microscopy
Tendons
Electrooptical effects
linear polarization
Modulators
electro-optics
rats
Laser beams
Rats
modulators

Keywords

  • Collagen
  • Phase-sensitive detection
  • Polarization modulation
  • Second harmonic generation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Stoller, P., Kim, B-M., Rubenchik, A. M., Reiser, K. M., & Da Silva, L. B. (2001). Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. In R. F. Haglund, J. Neev, & R. F. Wood (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4276, pp. 11-16) https://doi.org/10.1117/12.428013

Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. / Stoller, P.; Kim, Beop-Min; Rubenchik, A. M.; Reiser, K. M.; Da Silva, L. B.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / R.F. Haglund; J. Neev; R.F. Wood. Vol. 4276 2001. p. 11-16.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stoller, P, Kim, B-M, Rubenchik, AM, Reiser, KM & Da Silva, LB 2001, Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. in RF Haglund, J Neev & RF Wood (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4276, pp. 11-16, Commercial and Biomedical Applications of Ultrashort Pulse Lasers; Laser Plasma Generation and Diagnostics, San Jose, CA, United States, 01/1/23. https://doi.org/10.1117/12.428013
Stoller P, Kim B-M, Rubenchik AM, Reiser KM, Da Silva LB. Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. In Haglund RF, Neev J, Wood RF, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4276. 2001. p. 11-16 https://doi.org/10.1117/12.428013
Stoller, P. ; Kim, Beop-Min ; Rubenchik, A. M. ; Reiser, K. M. ; Da Silva, L. B. / Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. Proceedings of SPIE - The International Society for Optical Engineering. editor / R.F. Haglund ; J. Neev ; R.F. Wood. Vol. 4276 2001. pp. 11-16
@inproceedings{5178b34345a04036af2cd84585c4916b,
title = "Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection",
abstract = "The measurement of the second order nonlinear susceptibility of collagen in various biological tissues has potential applications in the detection of structural changes which are related to different pathological conditions. We investigate second harmonic generation in rat-tail tendon, a highly organized collagen structure consisting of parallel fibers. Using an electro-optic modulator and a quarter-wave plate, we modulate the linear polarization of an ultra-short pulse laser beam that is used to measure second harmonic generation (SHG) in a confocal microscopy setup. Phase-sensitive detection of the generated signal, coupled with a simple model of the collagen protein structures, allows us to measure a parameter γ related to nonlinear susceptibility and to determine the relative orientation of the structures. Our preliminary results indicate that it may be possible to use this parameter to characterize the structure.",
keywords = "Collagen, Phase-sensitive detection, Polarization modulation, Second harmonic generation",
author = "P. Stoller and Beop-Min Kim and Rubenchik, {A. M.} and Reiser, {K. M.} and {Da Silva}, {L. B.}",
year = "2001",
doi = "10.1117/12.428013",
language = "English",
volume = "4276",
pages = "11--16",
editor = "R.F. Haglund and J. Neev and R.F. Wood",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection

AU - Stoller, P.

AU - Kim, Beop-Min

AU - Rubenchik, A. M.

AU - Reiser, K. M.

AU - Da Silva, L. B.

PY - 2001

Y1 - 2001

N2 - The measurement of the second order nonlinear susceptibility of collagen in various biological tissues has potential applications in the detection of structural changes which are related to different pathological conditions. We investigate second harmonic generation in rat-tail tendon, a highly organized collagen structure consisting of parallel fibers. Using an electro-optic modulator and a quarter-wave plate, we modulate the linear polarization of an ultra-short pulse laser beam that is used to measure second harmonic generation (SHG) in a confocal microscopy setup. Phase-sensitive detection of the generated signal, coupled with a simple model of the collagen protein structures, allows us to measure a parameter γ related to nonlinear susceptibility and to determine the relative orientation of the structures. Our preliminary results indicate that it may be possible to use this parameter to characterize the structure.

AB - The measurement of the second order nonlinear susceptibility of collagen in various biological tissues has potential applications in the detection of structural changes which are related to different pathological conditions. We investigate second harmonic generation in rat-tail tendon, a highly organized collagen structure consisting of parallel fibers. Using an electro-optic modulator and a quarter-wave plate, we modulate the linear polarization of an ultra-short pulse laser beam that is used to measure second harmonic generation (SHG) in a confocal microscopy setup. Phase-sensitive detection of the generated signal, coupled with a simple model of the collagen protein structures, allows us to measure a parameter γ related to nonlinear susceptibility and to determine the relative orientation of the structures. Our preliminary results indicate that it may be possible to use this parameter to characterize the structure.

KW - Collagen

KW - Phase-sensitive detection

KW - Polarization modulation

KW - Second harmonic generation

UR - http://www.scopus.com/inward/record.url?scp=0034871923&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034871923&partnerID=8YFLogxK

U2 - 10.1117/12.428013

DO - 10.1117/12.428013

M3 - Conference contribution

VL - 4276

SP - 11

EP - 16

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Haglund, R.F.

A2 - Neev, J.

A2 - Wood, R.F.

ER -