Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection

P. Stoller, Beop-Min Kim, A. M. Rubenchik, K. M. Reiser, L. B. Da Silva

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

The measurement of the second order nonlinear susceptibility of collagen in various biological tissues has potential applications in the detection of structural changes which are related to different pathological conditions. We investigate second harmonic generation in rat-tail tendon, a highly organized collagen structure consisting of parallel fibers. Using an electro-optic modulator and a quarter-wave plate, we modulate the linear polarization of an ultra-short pulse laser beam that is used to measure second harmonic generation (SHG) in a confocal microscopy setup. Phase-sensitive detection of the generated signal, coupled with a simple model of the collagen protein structures, allows us to measure a parameter γ related to nonlinear susceptibility and to determine the relative orientation of the structures. Our preliminary results indicate that it may be possible to use this parameter to characterize the structure.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.F. Haglund, J. Neev, R.F. Wood
Pages11-16
Number of pages6
Volume4276
DOIs
Publication statusPublished - 2001
Externally publishedYes
EventCommercial and Biomedical Applications of Ultrashort Pulse Lasers; Laser Plasma Generation and Diagnostics - San Jose, CA, United States
Duration: 2001 Jan 232001 Jan 23

Other

OtherCommercial and Biomedical Applications of Ultrashort Pulse Lasers; Laser Plasma Generation and Diagnostics
CountryUnited States
CitySan Jose, CA
Period01/1/2301/1/23

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Keywords

  • Collagen
  • Phase-sensitive detection
  • Polarization modulation
  • Second harmonic generation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Stoller, P., Kim, B-M., Rubenchik, A. M., Reiser, K. M., & Da Silva, L. B. (2001). Measurement of the second order non-linear susceptibility of collagen using polarization modulation and phase-sensitive detection. In R. F. Haglund, J. Neev, & R. F. Wood (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4276, pp. 11-16) https://doi.org/10.1117/12.428013