Measurements of red blood cell deformability using a laser-diffraction slit rheometer

Sehyun Shin, Yunhee Ku, Myungsoo Park, Juhee Jang, Jangsoo Suh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The laser-diffraction technique has been applied to design a slit rheometer for measuring red blood cell deformability over a range of shear stress. Flow-rate and pressure-drop measurements are replaced with a measurement of pressure variation with time. Using a precision pressure transducer, one can measure the variation of pressure in the vacuum chamber, P(t), from which the stress and shear rate are mathematically calculated. In addition, a laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. The Elongation Index(EI) as a measure of RBC deformability is determined from an isointensity curve in the diffraction pattern sing an ellipse-fitting program. The advantages of the design are simplicity, i.e., ease of operation and no moving parts, low cost, short operating time, and the disposable kit which is contacted with blood sample.

Original languageEnglish
Title of host publicationProceedings of the IASTED International Conference on Biomedical Engineering
EditorsB. Tilg
Pages565-568
Number of pages4
Publication statusPublished - 2004
EventProceedings of the IASTED International Conference on Biomedical Engineering - Innsbruck, Austria
Duration: 2004 Feb 162004 Feb 18

Publication series

NameProceedings of the IASTED International Conference on Biomedical Engineering

Other

OtherProceedings of the IASTED International Conference on Biomedical Engineering
CountryAustria
CityInnsbruck
Period04/2/1604/2/18

Keywords

  • Laser diffraction
  • RBC deformability
  • Shear stress
  • Slit rheometer

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Shin, S., Ku, Y., Park, M., Jang, J., & Suh, J. (2004). Measurements of red blood cell deformability using a laser-diffraction slit rheometer. In B. Tilg (Ed.), Proceedings of the IASTED International Conference on Biomedical Engineering (pp. 565-568). [417-081] (Proceedings of the IASTED International Conference on Biomedical Engineering).