Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy

Chi Hoon Kim, Dae Gon Woo, Ji Hyung Park, Ok Hee Jeon, Seung Hak Lee, Chi Hyun Kim, Dae Sung Yoon, Han Sung Kim

Research output: Contribution to journalArticle

Abstract

The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Volume7
Issue number4
Publication statusPublished - 2010 Aug 1
Externally publishedYes

Fingerprint

Atomic Force Microscopy
Hormones
Chondrocytes
Osteoarthritis
Osteoporosis
Rats
Atomic force microscopy
Knee
Tomography
Knee Joint
Femur
Sprague Dawley Rats
Stiffness

Keywords

  • Atomic force microscopy (AFM)
  • Chondrocyte
  • Micro-CT
  • Osteoarthritis
  • Osteoporosis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Kim, C. H., Woo, D. G., Park, J. H., Jeon, O. H., Lee, S. H., Kim, C. H., ... Kim, H. S. (2010). Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy. Tissue Engineering and Regenerative Medicine, 7(4), 381-387.

Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy. / Kim, Chi Hoon; Woo, Dae Gon; Park, Ji Hyung; Jeon, Ok Hee; Lee, Seung Hak; Kim, Chi Hyun; Yoon, Dae Sung; Kim, Han Sung.

In: Tissue Engineering and Regenerative Medicine, Vol. 7, No. 4, 01.08.2010, p. 381-387.

Research output: Contribution to journalArticle

Kim, Chi Hoon ; Woo, Dae Gon ; Park, Ji Hyung ; Jeon, Ok Hee ; Lee, Seung Hak ; Kim, Chi Hyun ; Yoon, Dae Sung ; Kim, Han Sung. / Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy. In: Tissue Engineering and Regenerative Medicine. 2010 ; Vol. 7, No. 4. pp. 381-387.
@article{685c3e2ff66441cea176d71696b18e1a,
title = "Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy",
abstract = "The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.",
keywords = "Atomic force microscopy (AFM), Chondrocyte, Micro-CT, Osteoarthritis, Osteoporosis",
author = "Kim, {Chi Hoon} and Woo, {Dae Gon} and Park, {Ji Hyung} and Jeon, {Ok Hee} and Lee, {Seung Hak} and Kim, {Chi Hyun} and Yoon, {Dae Sung} and Kim, {Han Sung}",
year = "2010",
month = "8",
day = "1",
language = "English",
volume = "7",
pages = "381--387",
journal = "Tissue Engineering and Regenerative Medicine",
issn = "1738-2696",
publisher = "Springer Science + Business Media",
number = "4",

}

TY - JOUR

T1 - Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy

AU - Kim, Chi Hoon

AU - Woo, Dae Gon

AU - Park, Ji Hyung

AU - Jeon, Ok Hee

AU - Lee, Seung Hak

AU - Kim, Chi Hyun

AU - Yoon, Dae Sung

AU - Kim, Han Sung

PY - 2010/8/1

Y1 - 2010/8/1

N2 - The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.

AB - The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.

KW - Atomic force microscopy (AFM)

KW - Chondrocyte

KW - Micro-CT

KW - Osteoarthritis

KW - Osteoporosis

UR - http://www.scopus.com/inward/record.url?scp=84884560477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884560477&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 381

EP - 387

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

SN - 1738-2696

IS - 4

ER -