Mechanical characterization of amyloid fibrils using coarse-grained normal mode analysis

Gwonchan Yoon, Jinhak Kwak, Jae In Kim, Sung Soo Na, Kilho Eom

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Recent experimental studies have shown that amyloid fibril formed by aggregation of β peptide exhibits excellent mechanical properties comparable to other protein materials such as actin filaments and microtubules. These excellent mechanical properties of amyloid fibrils are related to their functional role in disease expression. This indicates the necessity of understanding how an amyloid fibril achieves the remarkable mechanical properties through self-aggregation with structural hierarchy. However, the structure-property-function relationship still remains elusive. In this work, the mechanical properties of human islet amyloid polypeptide (hIAPP) are studied with respect to its structural hierarchies and structural shapes by coarse-grained normal mode analysis. The simulation shows that hIAPP fibril can achieve the excellent bending rigidity via specific aggregation patterns such as antiparallel stacking of β peptides. Moreover, the length-dependent mechanical properties of amyloids are found. This length-dependent property has been elucidated from a Timoshenko beam model that takes into account the shear effect on the bending of amyloids. In summary, the study sheds light on the importance of not only the molecular architecture, which encodes the mechanical properties of the fibril, but also the shear effect on the mechanical (bending) behavior of the fibril.

Original languageEnglish
Pages (from-to)3454-3463
Number of pages10
JournalAdvanced Functional Materials
Volume21
Issue number18
DOIs
Publication statusPublished - 2011 Sep 23

Fingerprint

Amyloid
mechanical properties
Mechanical properties
Islet Amyloid Polypeptide
Agglomeration
polypeptides
Polypeptides
hierarchies
peptides
Peptides
shear
Timoshenko beams
rigidity
Rigidity
Actins
filaments
proteins
Proteins
simulation

Keywords

  • Amyloid fibrils
  • mechanical testing
  • Normal Mode Analysis

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Mechanical characterization of amyloid fibrils using coarse-grained normal mode analysis. / Yoon, Gwonchan; Kwak, Jinhak; Kim, Jae In; Na, Sung Soo; Eom, Kilho.

In: Advanced Functional Materials, Vol. 21, No. 18, 23.09.2011, p. 3454-3463.

Research output: Contribution to journalArticle

Yoon, Gwonchan ; Kwak, Jinhak ; Kim, Jae In ; Na, Sung Soo ; Eom, Kilho. / Mechanical characterization of amyloid fibrils using coarse-grained normal mode analysis. In: Advanced Functional Materials. 2011 ; Vol. 21, No. 18. pp. 3454-3463.
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