Abstract
The temporal response of young rabbit chondrocyte metabolism (including biosynthesis of extracellular matrix macromolecules such as collagen and aggrecan, both of which are essential components of normal cartilage tissue, and their messenger ribonucleic acid (mRNA) expression) in microporous elastomeric scaffolds made of poly(L-lactide-co-ε-caprolactone) subjected to different compressive regimes (loading frequency, loading duration per cycle, loading period, and continuous or intermittent compression) were studied over a 6-day culture period at 10% of compressive strain. A continuous dynamic compression improved the production of sulfated glycosaminoglycan (S-GAG), most of which was released into the culture medium upon loading. High mRNA expression of type II collagen was exhibited at a frequency of 0.1 Hz. Little frequency dependency was observed for aggrecan. An intermittent loading (24-h cycle of loading and unloading) or short loading and unloading duration per cycle-compression regime maintained high levels of mRNA expression. This strongly suggests that well-controlled mechanical conditioning regimes may control the gene expression of key metabolic substances relevant to functional cartilage tissue while the degree of release of these substances into the culture medium is minimized.
| Original language | English |
|---|---|
| Pages (from-to) | 29-40 |
| Number of pages | 12 |
| Journal | Tissue Engineering |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Cell Biology
- Biotechnology
Cite this
Mechanical loading-dependence of mRNA expressions of extracellular matrices of chondrocytes inoculated into elastomeric microporous poly(L-lactide-co- ε-caprolactone) scaffold. / Xie, Jun; Han, Zhiyi; Kim, Soo Hyun; Kim, Young Ha; Matsuda, Takehisa.
In: Tissue Engineering, Vol. 13, No. 1, 01.01.2007, p. 29-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanical loading-dependence of mRNA expressions of extracellular matrices of chondrocytes inoculated into elastomeric microporous poly(L-lactide-co- ε-caprolactone) scaffold
AU - Xie, Jun
AU - Han, Zhiyi
AU - Kim, Soo Hyun
AU - Kim, Young Ha
AU - Matsuda, Takehisa
PY - 2007/1/1
Y1 - 2007/1/1
N2 - The temporal response of young rabbit chondrocyte metabolism (including biosynthesis of extracellular matrix macromolecules such as collagen and aggrecan, both of which are essential components of normal cartilage tissue, and their messenger ribonucleic acid (mRNA) expression) in microporous elastomeric scaffolds made of poly(L-lactide-co-ε-caprolactone) subjected to different compressive regimes (loading frequency, loading duration per cycle, loading period, and continuous or intermittent compression) were studied over a 6-day culture period at 10% of compressive strain. A continuous dynamic compression improved the production of sulfated glycosaminoglycan (S-GAG), most of which was released into the culture medium upon loading. High mRNA expression of type II collagen was exhibited at a frequency of 0.1 Hz. Little frequency dependency was observed for aggrecan. An intermittent loading (24-h cycle of loading and unloading) or short loading and unloading duration per cycle-compression regime maintained high levels of mRNA expression. This strongly suggests that well-controlled mechanical conditioning regimes may control the gene expression of key metabolic substances relevant to functional cartilage tissue while the degree of release of these substances into the culture medium is minimized.
AB - The temporal response of young rabbit chondrocyte metabolism (including biosynthesis of extracellular matrix macromolecules such as collagen and aggrecan, both of which are essential components of normal cartilage tissue, and their messenger ribonucleic acid (mRNA) expression) in microporous elastomeric scaffolds made of poly(L-lactide-co-ε-caprolactone) subjected to different compressive regimes (loading frequency, loading duration per cycle, loading period, and continuous or intermittent compression) were studied over a 6-day culture period at 10% of compressive strain. A continuous dynamic compression improved the production of sulfated glycosaminoglycan (S-GAG), most of which was released into the culture medium upon loading. High mRNA expression of type II collagen was exhibited at a frequency of 0.1 Hz. Little frequency dependency was observed for aggrecan. An intermittent loading (24-h cycle of loading and unloading) or short loading and unloading duration per cycle-compression regime maintained high levels of mRNA expression. This strongly suggests that well-controlled mechanical conditioning regimes may control the gene expression of key metabolic substances relevant to functional cartilage tissue while the degree of release of these substances into the culture medium is minimized.
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UR - http://www.scopus.com/inward/citedby.url?scp=33846562419&partnerID=8YFLogxK
U2 - 10.1089/ten.2006.0060
DO - 10.1089/ten.2006.0060
M3 - Article
C2 - 17518579
AN - SCOPUS:33846562419
VL - 13
SP - 29
EP - 40
JO - Tissue Engineering
JF - Tissue Engineering
SN - 1076-3279
IS - 1
ER -