Mechanical properties and cell-culture characteristics of a polycaprolactone kagome-structure scaffold fabricated by a precision extruding deposition system

Se Hwan Lee, Yong Sang Cho, Myoung Wha Hong, Bu Kyu Lee, Yongdoo Park, Sang Hyug Park, Young Yul Kim, Young Sam Cho

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To enhance the mechanical properties of three-dimensional (3D) scaffolds used for bone regeneration in tissue engineering, many researchers have studied their structure and chemistry. In the structural engineering field, the kagome structure has been known to have an excellent relative strength. In this study, to enhance the mechanical properties of a synthetic polymer scaffold used for tissue engineering, we applied the 3D kagome structure to a porous scaffold for bone regeneration. Prior to fabricating the biocompatible-polymer scaffold, the ideal kagome structure, which was manufactured by a 3D printer of the digital light processing type, was compared with a grid-structure, which was used as the control group, using a compressive experiment. A polycaprolactone (PCL) kagome-structure scaffold was successfully fabricated by additive manufacturing using a 3D printer with a precision extruding deposition head. To assess the physical characteristics of the fabricated PCL-kagome-structure scaffold, we analyzed its porosity, pore size, morphological structure, surface roughness, compressive stiffness, and mechanical bending properties. The results showed that, the mechanical properties of proposed kagome-structure scaffold were superior to those of a grid-structure scaffold. Moreover, Sarcoma osteogenic (Saos-2) cells were used to evaluate the characteristics of in vitro cell proliferation. We carried out cell counting kit-8 (CCK-8) and DNA contents assays. Consequently, the cell proliferation of the kagome-structure scaffold was increased; this could be because the surface roughness of the kagome-structure scaffold enhances initial cell attachment.

Original languageEnglish
Article number055003
JournalBiomedical Materials (Bristol)
Volume12
Issue number5
DOIs
Publication statusPublished - 2017 Sep 13

Fingerprint

Polycaprolactone
Scaffolds (biology)
Cell culture
Scaffolds
Bone Regeneration
Cell Culture Techniques
Tissue Engineering
3D printers
Mechanical properties
Polymers
Cell Proliferation
Porosity
Osteosarcoma
Cell proliferation
Tissue engineering
Head
Research Personnel
Bone
Light
Surface roughness

Keywords

  • 3D printing
  • kagome
  • polycaprolactone
  • precision extruding deposition
  • scaffold

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Mechanical properties and cell-culture characteristics of a polycaprolactone kagome-structure scaffold fabricated by a precision extruding deposition system. / Lee, Se Hwan; Cho, Yong Sang; Hong, Myoung Wha; Lee, Bu Kyu; Park, Yongdoo; Park, Sang Hyug; Kim, Young Yul; Cho, Young Sam.

In: Biomedical Materials (Bristol), Vol. 12, No. 5, 055003, 13.09.2017.

Research output: Contribution to journalArticle

Lee, Se Hwan ; Cho, Yong Sang ; Hong, Myoung Wha ; Lee, Bu Kyu ; Park, Yongdoo ; Park, Sang Hyug ; Kim, Young Yul ; Cho, Young Sam. / Mechanical properties and cell-culture characteristics of a polycaprolactone kagome-structure scaffold fabricated by a precision extruding deposition system. In: Biomedical Materials (Bristol). 2017 ; Vol. 12, No. 5.
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