Mechanical property characterization of the zebrafish embryo chorion

Deok Ho Kim, Yu Sun, Seok Yun, Byungkyu Kim, Chang Nam Hwang, Sang Ho Lee, Bradley J. Nelson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

33 Citations (Scopus)

Abstract

A microrobotic force sensing system is used to characterize the mechanical properties of the chorion of zebrafish embryos and quantitate the mechanical property differences of the chorion at different developmental stages. Quantitative relationships between applied forces and chorion structural deformations are established for various developmental stages. The measured penetration forces for puncturing chorion at the blastula stage are 1.3 times as large as those at the pre-hatching stage. Through the use of an analytical biomembrane elastic model, chorion elastic modulus values are determined. The modulus at the blastula stage is 1.66 times as large as that at the pre-hatching stage. The experimental results quantitatively describe "chorion softening," which is mostly due to the proteolytic activity at the pre-hatching stage. This study also reveals the effect of pronase treatment on zebrafish chorion, which produces an "artificial chorion softening" effect. This draws an analogy between pronase treatment and proteolytic activities at the pre-hatching stage.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages5061-5064
Number of pages4
Volume26 VII
Publication statusPublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: 2004 Sep 12004 Sep 5

Other

OtherConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004
CountryUnited States
CitySan Francisco, CA
Period04/9/104/9/5

Keywords

  • Cellular force sensing
  • Chorion softening
  • Elastic modulus
  • Microrobotic cell manipulation
  • Zebrafish

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Kim, D. H., Sun, Y., Yun, S., Kim, B., Hwang, C. N., Lee, S. H., & Nelson, B. J. (2004). Mechanical property characterization of the zebrafish embryo chorion. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 26 VII, pp. 5061-5064)