Mechanisms of patulin toxicity under conditions that inhibit yeast growth

Yumiko Iwahashi, Hiroshi Hosoda, Ji Hyun Park, Joo Hee Lee, Yoshiteru Suzuki, Emiko Kitagawa, Satomi Mizukami Murata, Nam Soo Jwa, Man Bock Gu, Hitoshi Iwahashi

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Patulin, 4-hydroxy-4H-furo[3,2c]pyran-2(6H)-one, is one of the best characterized and most widely disseminated mycotoxins found in agricultural products. Nonetheless, the mechanisms by which patulin causes toxicity are not well understood. Thus, the cytotoxicity of patulin was characterized by analysis of the yeast transcriptome upon challenge with patulin. Interestingly, patulin-induced yeast gene expression profiles were found to be similar to gene expression patterns obtained after treatment with the antifungal agricultural chemicals thiuram, maneb, and zineb. Moreover, patulin treatment was found to activate protein degradation, especially proteasome activities, sulfur amino acid metabolism, and the defense system for oxidative stress. Damage to DNA by alkylation was also suggested, and this seemed to be repaired by recombinational and excision repair mechanisms. Furthermore, the results provide potential biomarker genes for the detection of patulin in agricultural products. The results suggest the possibility of applying the yeast transcriptome system for the evaluation of chemicals, especially for natural chemicals that are difficult to get by organic synthesis.

Original languageEnglish
Pages (from-to)1936-1942
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number5
DOIs
Publication statusPublished - 2006 Mar 8

Fingerprint

Patulin
patulin
Yeast
Toxicity
Yeasts
yeasts
toxicity
Growth
Agricultural products
agricultural products
Transcriptome
Gene expression
transcriptome
Zineb
Maneb
zineb
Thiram
maneb
Agrochemicals
Sulfur Amino Acids

Keywords

  • DNA microarry
  • Mutagenesis
  • Mycotoxin
  • Patulin
  • Yeast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Iwahashi, Y., Hosoda, H., Park, J. H., Lee, J. H., Suzuki, Y., Kitagawa, E., ... Iwahashi, H. (2006). Mechanisms of patulin toxicity under conditions that inhibit yeast growth. Journal of Agricultural and Food Chemistry, 54(5), 1936-1942. https://doi.org/10.1021/jf052264g

Mechanisms of patulin toxicity under conditions that inhibit yeast growth. / Iwahashi, Yumiko; Hosoda, Hiroshi; Park, Ji Hyun; Lee, Joo Hee; Suzuki, Yoshiteru; Kitagawa, Emiko; Murata, Satomi Mizukami; Jwa, Nam Soo; Gu, Man Bock; Iwahashi, Hitoshi.

In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 5, 08.03.2006, p. 1936-1942.

Research output: Contribution to journalArticle

Iwahashi, Y, Hosoda, H, Park, JH, Lee, JH, Suzuki, Y, Kitagawa, E, Murata, SM, Jwa, NS, Gu, MB & Iwahashi, H 2006, 'Mechanisms of patulin toxicity under conditions that inhibit yeast growth', Journal of Agricultural and Food Chemistry, vol. 54, no. 5, pp. 1936-1942. https://doi.org/10.1021/jf052264g
Iwahashi Y, Hosoda H, Park JH, Lee JH, Suzuki Y, Kitagawa E et al. Mechanisms of patulin toxicity under conditions that inhibit yeast growth. Journal of Agricultural and Food Chemistry. 2006 Mar 8;54(5):1936-1942. https://doi.org/10.1021/jf052264g
Iwahashi, Yumiko ; Hosoda, Hiroshi ; Park, Ji Hyun ; Lee, Joo Hee ; Suzuki, Yoshiteru ; Kitagawa, Emiko ; Murata, Satomi Mizukami ; Jwa, Nam Soo ; Gu, Man Bock ; Iwahashi, Hitoshi. / Mechanisms of patulin toxicity under conditions that inhibit yeast growth. In: Journal of Agricultural and Food Chemistry. 2006 ; Vol. 54, No. 5. pp. 1936-1942.
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