Mechanistic examination of protein release from polymer nanofibers

M. Gandhi, R. Srikar, A. L. Yarin, C. M. Megaridis, R. A. Gemeinhart

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Therapeutic proteins have emerged as a significant class of pharmaceutical agents over the past several decades. The potency, rapid elimination, and systemic side effects have prompted the need of spatiotemporally controlled release for proteins maybe more than any other active therapeutic molecules. This work examines the release of two model protein compounds, bovine serum albumin (BSA) and an anti-integrin antibody (AI), from electrospun polyca-prolactone (PCL) nanofiber mats. The anti-integrin antibody was chosen as a model of antibody therapy; in particular, anti-integrin antibodies are a promising class of therapeutic molecules for cancer and angiogenic diseases. The release kinetics were studied experimentally and interpreted in the framework of a recently published theory of desorption-limited drug release from nondegrading-or very slowly degrading-fibers. The results are consistent with a protein release mechanism dominated by desorption from the polymer surface, while the polycaprolactone nanofibers are not degrading at an appreciable rate.

Original languageEnglish
Pages (from-to)641-647
Number of pages7
JournalMolecular Pharmaceutics
Volume6
Issue number2
DOIs
Publication statusPublished - 2009 Apr 6

Keywords

  • Controlled release
  • Modeling
  • Nanofiber
  • Protein

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

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  • Cite this

    Gandhi, M., Srikar, R., Yarin, A. L., Megaridis, C. M., & Gemeinhart, R. A. (2009). Mechanistic examination of protein release from polymer nanofibers. Molecular Pharmaceutics, 6(2), 641-647. https://doi.org/10.1021/mp800160p