Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology

Na Kyoung Lee, Yeo Lang Park, Ga Jin Choe, Hyo-Ihl Chang, Hyun Dong Paik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl 2, MgSO 4, KH 2PO 4, (NH 4) 2SO 4, FeSO 4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalKorean Journal for Food Science of Animal Resources
Volume30
Issue number3
Publication statusPublished - 2010 Jun 1

Keywords

  • Chicken feces
  • Lactobacillus acidophilus
  • Medium optimization
  • Probiotics
  • Response surface methodology

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

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