Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology

Na Kyoung Lee, Yeo Lang Park, Ga Jin Choe, Hyo-Ihl Chang, Hyun Dong Paik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl 2, MgSO 4, KH 2PO 4, (NH 4) 2SO 4, FeSO 4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalKorean Journal for Food Science of Animal Resources
Volume30
Issue number3
Publication statusPublished - 2010 Jun 1

Fingerprint

Lactobacillus acidophilus
Probiotics
Lactose
yeast extract
response surface methodology
probiotics
lactose
inorganic salts
compound A 12
Yeasts
Nitrogen
Carbon
Salts
beef extracts
malt extract
carbon
Livestock
Mannitol
nitrogen
Fructose

Keywords

  • Chicken feces
  • Lactobacillus acidophilus
  • Medium optimization
  • Probiotics
  • Response surface methodology

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

Cite this

Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology. / Lee, Na Kyoung; Park, Yeo Lang; Choe, Ga Jin; Chang, Hyo-Ihl; Paik, Hyun Dong.

In: Korean Journal for Food Science of Animal Resources, Vol. 30, No. 3, 01.06.2010, p. 359-364.

Research output: Contribution to journalArticle

@article{e898637c4d8b4dd59ee8df8ae7c0312f,
title = "Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology",
abstract = "Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl 2, MgSO 4, KH 2PO 4, (NH 4) 2SO 4, FeSO 4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.",
keywords = "Chicken feces, Lactobacillus acidophilus, Medium optimization, Probiotics, Response surface methodology",
author = "Lee, {Na Kyoung} and Park, {Yeo Lang} and Choe, {Ga Jin} and Hyo-Ihl Chang and Paik, {Hyun Dong}",
year = "2010",
month = "6",
day = "1",
language = "English",
volume = "30",
pages = "359--364",
journal = "Korean Journal for Food Science of Animal Resources",
issn = "1225-8563",
publisher = "Korean Society for Food Science of Animal Resources",
number = "3",

}

TY - JOUR

T1 - Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology

AU - Lee, Na Kyoung

AU - Park, Yeo Lang

AU - Choe, Ga Jin

AU - Chang, Hyo-Ihl

AU - Paik, Hyun Dong

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl 2, MgSO 4, KH 2PO 4, (NH 4) 2SO 4, FeSO 4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

AB - Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl 2, MgSO 4, KH 2PO 4, (NH 4) 2SO 4, FeSO 4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

KW - Chicken feces

KW - Lactobacillus acidophilus

KW - Medium optimization

KW - Probiotics

KW - Response surface methodology

UR - http://www.scopus.com/inward/record.url?scp=79960760183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960760183&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79960760183

VL - 30

SP - 359

EP - 364

JO - Korean Journal for Food Science of Animal Resources

JF - Korean Journal for Food Science of Animal Resources

SN - 1225-8563

IS - 3

ER -