Membrane of hybrid chitosan-silica xerogel for guided bone regeneration

Eun Jung Lee, Du S. Shin, Hyoun E. Kim, Hae W. Kim, Young-Hag Koh, Jun Hyeog Jang

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

Chitosan-silica xerogel hybrid membranes were fabricated using a sol-gel process and their potential applications in guided bone regeneration (GBR) were investigated in terms of their in vitro cellular activity and in vivo bone regeneration ability. TEM observation revealed that the silica xerogel was dispersed in the chitosan matrix on the nanoscale. The hybrid membrane showed superior mechanical properties to chitosan in the wet state and the rapid induction of calcium phosphate minerals in simulated body fluid, reflecting its excellent in vitro bone bioactivity. Osteoblastic cells were observed to adhere well and grow actively on the hybrid membrane to a level higher than that observed on the chitosan membrane. The alkaline phosphatase activity of the cells was also much higher on the hybrid than on the chitosan membrane. The in vivo study in a rat calvarial model demonstrated significantly enhanced bone regeneration using the hybrid membrane compared to that observed using the pure chitosan one. Histomorphometric analysis performed 3 weeks after implantation revealed a fully closed defect in the hybrid membrane, whereas there was only 57% defect closure in the chitosan membrane.

Original languageEnglish
Pages (from-to)743-750
Number of pages8
JournalBiomaterials
Volume30
Issue number5
DOIs
Publication statusPublished - 2009 Feb 1

Fingerprint

Xerogels
Bone Regeneration
Chitosan
Silicon Dioxide
Bone
Silica
Membranes
Phosphate minerals
Defects
Body fluids
Phosphatases
Calcium phosphate
Body Fluids
Polymethyl Methacrylate
Bioactivity
Sol-gel process
Minerals
Alkaline Phosphatase
Rats
Gels

Keywords

  • Chitosan
  • Guided bone regeneration
  • Hybrids
  • In vivo bone formation
  • Silica xerogel

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites

Cite this

Membrane of hybrid chitosan-silica xerogel for guided bone regeneration. / Lee, Eun Jung; Shin, Du S.; Kim, Hyoun E.; Kim, Hae W.; Koh, Young-Hag; Jang, Jun Hyeog.

In: Biomaterials, Vol. 30, No. 5, 01.02.2009, p. 743-750.

Research output: Contribution to journalArticle

Lee, Eun Jung ; Shin, Du S. ; Kim, Hyoun E. ; Kim, Hae W. ; Koh, Young-Hag ; Jang, Jun Hyeog. / Membrane of hybrid chitosan-silica xerogel for guided bone regeneration. In: Biomaterials. 2009 ; Vol. 30, No. 5. pp. 743-750.
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