TY - JOUR
T1 - Mesophilic biogenic H2 production using galactose in a fixed bed reactor
AU - Sivagurunathan, Periyasamy
AU - Anburajan, Parthiban
AU - Park, Jong Hun
AU - Kumar, Gopalakrishnan
AU - Park, Hee Deung
AU - Kim, Sang Hyoun
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2014R1A2A2A04005475).
Publisher Copyright:
© 2016 Hydrogen Energy Publications LLC
PY - 2017/2/9
Y1 - 2017/2/9
N2 - This study investigated hydrogen fermentation from galactose in a fixed bed reactor (FBR) under a mesophilic temperature of 37 °C. The fixed bed reactor was packed with Lantec HD-Q-PAC material to support biomass growth and microbial assisted granule formation over the time course of the reactor operation. Hydraulic retention times (HRT) of 1.5–12 h over 79 days of bioreactor operation were assessed to determine the optimal hydrogen production efficiency of the system. The maximum hydrogen production rate and hydrogen yield of 65.5 L/L/d and 2.60 mol/mol hexoseaddedwere achieved at a hydraulic retention time (HRT) of 2 h, which is higher than the reported maximum hydrogen production performance of reactors fed with galactose using other reactor configuration. The major volatile fatty acids formed were butyric and acetic acids. Microbial community analysis by quantitative real time polymerase chain reaction revealed that population changes greatly affected hydrogen production performance. The increase in the bacterial fraction of Lactobacillus spp. over 21.5% at a 1.5 h HRT led to increased lactic acid production up to 1865 mg/L, which deteriorated the reactor performances. Conversely, a bacterial fraction of Clostridium butyricum over 98% was observed when the H2production performance and organic acid distribution were recovered using a 2 h HRT. FBR would be a promising reactor configuration to achieve high hydrogen productivity while preventing the wash out of active biomass.
AB - This study investigated hydrogen fermentation from galactose in a fixed bed reactor (FBR) under a mesophilic temperature of 37 °C. The fixed bed reactor was packed with Lantec HD-Q-PAC material to support biomass growth and microbial assisted granule formation over the time course of the reactor operation. Hydraulic retention times (HRT) of 1.5–12 h over 79 days of bioreactor operation were assessed to determine the optimal hydrogen production efficiency of the system. The maximum hydrogen production rate and hydrogen yield of 65.5 L/L/d and 2.60 mol/mol hexoseaddedwere achieved at a hydraulic retention time (HRT) of 2 h, which is higher than the reported maximum hydrogen production performance of reactors fed with galactose using other reactor configuration. The major volatile fatty acids formed were butyric and acetic acids. Microbial community analysis by quantitative real time polymerase chain reaction revealed that population changes greatly affected hydrogen production performance. The increase in the bacterial fraction of Lactobacillus spp. over 21.5% at a 1.5 h HRT led to increased lactic acid production up to 1865 mg/L, which deteriorated the reactor performances. Conversely, a bacterial fraction of Clostridium butyricum over 98% was observed when the H2production performance and organic acid distribution were recovered using a 2 h HRT. FBR would be a promising reactor configuration to achieve high hydrogen productivity while preventing the wash out of active biomass.
KW - Fixed bed reactor
KW - Galactose
KW - High-rate
KW - Hydraulic retention time
UR - http://www.scopus.com/inward/record.url?scp=84995566517&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2016.07.203
DO - 10.1016/j.ijhydene.2016.07.203
M3 - Article
AN - SCOPUS:84995566517
VL - 42
SP - 3658
EP - 3666
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 6
ER -