Metabolic Design of Corynebacterium glutamicum for Production of l -Cysteine with Consideration of Sulfur-Supplemented Animal Feed

Young Chul Joo, Jeong Eun Hyeon, Sung Ok Han

Research output: Contribution to journalArticle

10 Citations (Scopus)


l-Cysteine is a valuable sulfur-containing amino acid widely used as a nutrition supplement in industrial food production, agriculture, and animal feed. However, this amino acid is mostly produced by acid hydrolysis and extraction from human or animal hairs. In this study, we constructed recombinant Corynebacterium glutamicum strains that overexpress combinatorial genes for l-cysteine production. The aims of this work were to investigate the effect of the combined overexpression of serine acetyltransferase (CysE), O-acetylserine sulfhydrylase (CysK), and the transcriptional regulator CysR on l-cysteine production. The CysR-overexpressing strain accumulated approximately 2.7-fold more intracellular sulfide than the control strain (empty pMT-tac vector). Moreover, in the resulting CysEKR recombinant strain, combinatorial overexpression of genes involved in l-cysteine production successfully enhanced its production by approximately 3.0-fold relative to that in the control strain. This study demonstrates a biotechnological model for the production of animal feed supplements such as l-cysteine using metabolically engineered C. glutamicum.

Original languageEnglish
Pages (from-to)4698-4707
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Issue number23
Publication statusPublished - 2017 Jun 14



  • animal feed supplements
  • Corynebacterium glutamicum
  • l -cysteine production
  • metabolic engineering
  • sulfur-containing amino acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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