Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical

Hee Taek Kim, Tae Uk Khang, Kei Anne Baritugo, Sung Min Hyun, Kyoung Hee Kang, Sol Hee Jung, Bong Keun Song, Kyungmoon Park, Min-Kyu Oh, Gi Bae Kim, Hyun Uk Kim, Sang Yup Lee, Si Jae Park, Jeong Chan Joo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. It was found that use of N-terminal His6-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of L-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.

Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalMetabolic Engineering
Volume51
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Codon optimization
  • Corynebacterium glutamicum
  • davTDBA
  • Fed-batch fermentation
  • Glutaric acid
  • His-tag
  • L‐Lysine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Kim, H. T., Khang, T. U., Baritugo, K. A., Hyun, S. M., Kang, K. H., Jung, S. H., Song, B. K., Park, K., Oh, M-K., Kim, G. B., Kim, H. U., Lee, S. Y., Park, S. J., & Joo, J. C. (2019). Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical. Metabolic Engineering, 51, 99-109. https://doi.org/10.1016/j.ymben.2018.08.007