Abstract
The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.
| Original language | English |
|---|---|
| Journal | Bioresource Technology |
| DOIs | |
| Publication status | Accepted/In press - 2017 Mar 31 |
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Keywords
- 2,3-Butanediol
- Enterobacter aerogenes
- Metabolic engineering
- Sugarcane bagasse
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate. / Um, Jaeyong; Kim, Duck Gyun; Jung, Moo Young; Saratale, Ganesh D.; Oh, Min-Kyu.
In: Bioresource Technology, 31.03.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate
AU - Um, Jaeyong
AU - Kim, Duck Gyun
AU - Jung, Moo Young
AU - Saratale, Ganesh D.
AU - Oh, Min-Kyu
PY - 2017/3/31
Y1 - 2017/3/31
N2 - The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.
AB - The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3-butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.
KW - 2,3-Butanediol
KW - Enterobacter aerogenes
KW - Metabolic engineering
KW - Sugarcane bagasse
UR - http://www.scopus.com/inward/record.url?scp=85020232848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020232848&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.05.166
DO - 10.1016/j.biortech.2017.05.166
M3 - Article
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -