Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Laxmi Prasad Thapa; Sang Jun Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.bej.2018.12.019

Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Laxmi Prasad Thapa, Sang Jun Lee, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.

Original language	English
Pages (from-to)	169-178
Number of pages	10
Journal	Biochemical Engineering Journal
Volume	143
DOIs	https://doi.org/10.1016/j.bej.2018.12.019
Publication status	Published - 2019 Mar 15

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Keywords

2,3-Butanediol
Biorefinery
Enterobacter aerogenes
Metabolic engineering

ASJC Scopus subject areas

Biotechnology
Bioengineering
Environmental Engineering
Biomedical Engineering

Cite this

Thapa, L. P., Lee, S. J., Park, C., & Kim, S. W. (2019). Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol. Biochemical Engineering Journal, 143, 169-178. https://doi.org/10.1016/j.bej.2018.12.019

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abstract = "The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.",

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10.1016/j.bej.2018.12.019