TY - JOUR
T1 - Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol
AU - Thapa, Laxmi Prasad
AU - Lee, Sang Jun
AU - Park, Chulhwan
AU - Kim, Seung Wook
N1 - Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2014R1A2A2A01007321 ) and the Ministry of Education ( 2016R1D1A1A09918327 ).
PY - 2019/3/15
Y1 - 2019/3/15
N2 - The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.
AB - The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.
KW - 2,3-Butanediol
KW - Biorefinery
KW - Enterobacter aerogenes
KW - Metabolic engineering
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U2 - 10.1016/j.bej.2018.12.019
DO - 10.1016/j.bej.2018.12.019
M3 - Article
AN - SCOPUS:85059461422
VL - 143
SP - 169
EP - 178
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -