TY - JOUR
T1 - Metabolic engineering of Klebsiella pneumoniae for the production of cis,cis-muconic acid
AU - Jung, Hwi Min
AU - Jung, Moo Young
AU - Oh, Min Kyu
N1 - Funding Information:
This work was supported by grants from the National Research Foundation of Korea funded by the Korean Government (2012M1A2A2026560 and 2014R1A2A2A03007094).
Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.
PY - 2015/6/28
Y1 - 2015/6/28
N2 - cis,cis-Muconic acid (ccMA), a metabolic intermediate of Klebsiella pneumoniae, can be converted to adipic acid and terephthalic acid, which are important monomers of synthetic polymers. However, wild-type K. pneumoniae does not produce ccMA because intracellular carbon flow does not favor ccMA biosynthesis. In this study, several metabolic engineering strategies were used in an attempt to modify the wild-type strain to induce it to produce ccMA. First, by blocking the synthesis of aromatic amino acids, 343 mg/L of catechol, a precursor of ccMA, was produced. Then, the native catechol 1,2-dioxygenasegene (catA) was overexpressed, which caused the strain to convert the catechol to ccMA. The production of ccMA was further improved by deletion of the muconate cycloisomerase gene (catB) and by deleting a feedback inhibitor of the aromatic amino acid pathway. Further improvement was achieved by adjusting the pH of the culture broth. The developed strain produced 2.1 g/L of ccMA in flask cultivation. The results showed the potential of K. pneumoniae as a ccMA producer.
AB - cis,cis-Muconic acid (ccMA), a metabolic intermediate of Klebsiella pneumoniae, can be converted to adipic acid and terephthalic acid, which are important monomers of synthetic polymers. However, wild-type K. pneumoniae does not produce ccMA because intracellular carbon flow does not favor ccMA biosynthesis. In this study, several metabolic engineering strategies were used in an attempt to modify the wild-type strain to induce it to produce ccMA. First, by blocking the synthesis of aromatic amino acids, 343 mg/L of catechol, a precursor of ccMA, was produced. Then, the native catechol 1,2-dioxygenasegene (catA) was overexpressed, which caused the strain to convert the catechol to ccMA. The production of ccMA was further improved by deletion of the muconate cycloisomerase gene (catB) and by deleting a feedback inhibitor of the aromatic amino acid pathway. Further improvement was achieved by adjusting the pH of the culture broth. The developed strain produced 2.1 g/L of ccMA in flask cultivation. The results showed the potential of K. pneumoniae as a ccMA producer.
KW - Aromatic amino acid pathway
KW - Benzoate degradation pathway
KW - Klebsiella pneumoniae
KW - cis,cis-Muconic acid
UR - http://www.scopus.com/inward/record.url?scp=84929943339&partnerID=8YFLogxK
U2 - 10.1007/s00253-015-6442-3
DO - 10.1007/s00253-015-6442-3
M3 - Article
C2 - 25681152
AN - SCOPUS:84929943339
VL - 99
SP - 5217
EP - 5225
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 12
ER -