Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides

Sushila Maharjan, Je Won Park, Yeo Joon Yoon, Hei Chan Lee, Jae Kyung Sohng

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalBiotechnology letters
Volume32
Issue number2
DOIs
Publication statusPublished - 2010 Jan
Externally publishedYes

Keywords

  • 1,3,6,8-tetrahydroxynaphthalene synthase
  • Acetyl-CoA carboxylase
  • Flaviolin
  • Streptomyces venezuelae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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