Metabolic profiling and biological mechanisms of body fat reduction in mice fed the ethanolic extract of black-colored rice

Hea Won Kim, A. Young Lee, Siok Koon Yeo, Hyun Chung, Ji Hae Lee, Minh Hien Hoang, Yaoyao Jia, Sang Ik Han, Sea Kwan Oh, Sung-Joon Lee, Young Suk Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We investigated the mechanisms underlying the antiobesogenic properties of black-colored rice (BCR; Oryza sativa L. cv. Heugkwangbyeo) with high levels of anthocyanins. Stimulation of cultured adipocytes with BCR extract caused a significant decrease in cellular lipid reduction, and C57BL/6J mice fed BCR extract for 8. weeks exhibited significant reductions in body weight and body fat. Plasma concentrations of leptin and insulin decreased, while levels of adiponectin increased in BCR group. These metabolic alterations were mediated by the activation of peroxisome proliferator-activated receptors (PPARs), activating PPAR-α while inhibiting PPAR-γ. These changes were accompanied by down-regulation and up-regulation of the genes involved in hepatic fatty acid synthesis and fatty acid oxidation, respectively. Evaluation of gene expression in adipocytes also suggested that those involved in lipid accumulation were reduced, with a concomitant induction of the genes involved in lipid hydrolysis. The plasma metabolites in mice rendered obese by intake of a high-fat-diet were analyzed using GC-TOF-MS and multivariate analysis after being fed different diets for 8. weeks: control, Garcinia cambogia, control rice (O. sativa L. cv. Ilpumbyeo), and BCR. The BCR group could be distinguished from the others after 4. weeks of the intervention using multivariate analysis. The major components related to this distinction were mannose, isoleucine, threonine, and phenylalanine. Also, body weight, glucose, and total cholesterol were positively correlated with the levels of propionic acid, butyric acid, cholesterol, alanine, ribitol, glucose, and galactose, whereas the high-density lipoprotein (HDL)/non-HDL ratio was negatively correlated with those of stearic acid, cholesterol, valine, leucine, glucose, and galactose.

Original languageEnglish
Pages (from-to)373-390
Number of pages18
JournalFood Research International
Volume53
Issue number1
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

metabolomics
body fat
Peroxisome Proliferator-Activated Receptors
Adipose Tissue
cholesterol
adipocytes
rice
galactose
glucose
multivariate analysis
Cholesterol
Oryza sativa
mice
extracts
Garcinia gummi-guta
lipids
Galactose
Lipids
Adipocytes
Glucose

Keywords

  • Antiobesity
  • Black colored rice
  • GC-TOF-MS
  • Metabolic profiling

ASJC Scopus subject areas

  • Food Science

Cite this

Metabolic profiling and biological mechanisms of body fat reduction in mice fed the ethanolic extract of black-colored rice. / Kim, Hea Won; Lee, A. Young; Yeo, Siok Koon; Chung, Hyun; Lee, Ji Hae; Hoang, Minh Hien; Jia, Yaoyao; Han, Sang Ik; Oh, Sea Kwan; Lee, Sung-Joon; Kim, Young Suk.

In: Food Research International, Vol. 53, No. 1, 01.08.2013, p. 373-390.

Research output: Contribution to journalArticle

Kim, Hea Won ; Lee, A. Young ; Yeo, Siok Koon ; Chung, Hyun ; Lee, Ji Hae ; Hoang, Minh Hien ; Jia, Yaoyao ; Han, Sang Ik ; Oh, Sea Kwan ; Lee, Sung-Joon ; Kim, Young Suk. / Metabolic profiling and biological mechanisms of body fat reduction in mice fed the ethanolic extract of black-colored rice. In: Food Research International. 2013 ; Vol. 53, No. 1. pp. 373-390.
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AU - Hoang, Minh Hien

AU - Jia, Yaoyao

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