Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria

Hyun Jeong Lee, Jigyeong Son, Sang Jun Sim, Han Min Woo

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Designing synthetic pathways for efficient CO2 fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO2 into acetyl-CoA-derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.

Original languageEnglish
Pages (from-to)1860-1868
Number of pages9
JournalPlant Biotechnology Journal
Issue number9
Publication statusPublished - 2020 Sep 1


  • CO conversion
  • cyanobacteria
  • metabolic engineering
  • synthetic pyruvate dehydrogenase bypass

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science


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