Abstract
Designing synthetic pathways for efficient CO2 fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO2 into acetyl-CoA-derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.
Original language | English |
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Pages (from-to) | 1860-1868 |
Number of pages | 9 |
Journal | Plant Biotechnology Journal |
Volume | 18 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2020 Sep 1 |
Keywords
- CO conversion
- cyanobacteria
- metabolic engineering
- synthetic pyruvate dehydrogenase bypass
ASJC Scopus subject areas
- Biotechnology
- Agronomy and Crop Science
- Plant Science