Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions

Donggeon Choi, Sae Bom Lee, Sohyun Kim, Byoungnam Min, In-Geol Choi, In Seop Chang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46. mM and 0.259. ±. 0.034. U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalBioresource Technology
Volume154
DOIs
Publication statusPublished - 2014 Feb 1

Keywords

  • Anaerobic conditions
  • Current generation
  • Glucose utilization
  • Microbial fuel cells
  • Shewanella oneidensis MR-1

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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