Metabolomic elucidation of the effects of media and carbon sources on fatty acid production by Yarrowia lipolytica

Eun Ju Yun, James Lee, Do Hyoung Kim, Jungyeon Kim, Sooah Kim, Yong Su Jin, Kyoung Heon Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lipid production by oleaginous Yarrowia lipolytica depends highly on culture environments, such as carbon sources, carbon/nitrogen (C/N) ratios, types of media, and cellular growth phases. In this study, the effects of media and carbon sources on lipid and metabolite production were investigated by profiling fatty acids and intracellular metabolites of Y. lipolytica grown in various media. The highest total fatty acid yield 114.04 ± 6.23 mg/g dry cell weight was achieved by Y. lipolytica grown in minimal medium with glycerol (SCG) in the exponential phase. The high lipid production by Y. lipolytica in SCG was presumed to be due to the higher C/N ratio in SCG than in the complex media. Moreover, glycerol promoted lipid production better than glucose in both complex and minimal media because glycerol can easily incorporate into the core of triglycerides. Metabolite profiling revealed that levels of long-chain fatty acids, such as stearic acid, palmitic acid, and arachidic acid, increased in SCG medium. Meanwhile, in complex media supplemented with either glucose or glycerol, levels of amino acids, such as cysteine, methionine, and glycine, highly increased. This metabolomic approach could be applied to modulate the global metabolic network of Y. lipolytica for producing lipids and other valuable products.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalJournal of Biotechnology
Volume272-273
DOIs
Publication statusPublished - 2018 Apr 20

Fingerprint

Yarrowia
Metabolomics
Glycerol
Fatty Acids
Carbon
Lipids
Nitrogen
Glucose
Palmitic Acid
Metabolic Networks and Pathways
Methionine
Glycine
Cysteine
Triglycerides
Amino Acids
Weights and Measures
Growth

Keywords

  • Glycerol
  • Lipogenesis
  • Media
  • Metabolomics
  • Yarrowia lipolytica

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Metabolomic elucidation of the effects of media and carbon sources on fatty acid production by Yarrowia lipolytica. / Yun, Eun Ju; Lee, James; Kim, Do Hyoung; Kim, Jungyeon; Kim, Sooah; Jin, Yong Su; Kim, Kyoung Heon.

In: Journal of Biotechnology, Vol. 272-273, 20.04.2018, p. 7-13.

Research output: Contribution to journalArticle

Yun, Eun Ju ; Lee, James ; Kim, Do Hyoung ; Kim, Jungyeon ; Kim, Sooah ; Jin, Yong Su ; Kim, Kyoung Heon. / Metabolomic elucidation of the effects of media and carbon sources on fatty acid production by Yarrowia lipolytica. In: Journal of Biotechnology. 2018 ; Vol. 272-273. pp. 7-13.
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