Metabolomic response of a marine bacterium to 3,6-anhydro-L-galactose, the rare sugar from red macroalgae, as the sole carbon source

Eun Ju Yun, Sora Yu, Sooah Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

Abstract

Marine red macroalgae have received much attention as sustainable resources for producing bio-based products. Therefore, understanding the metabolic pathways of carbohydrates from red macroalgae, in fermentative microorganisms, is crucial for efficient bioconversion of the carbohydrates into bio-based products. Recently, the novel catabolic pathway of 3,6-anhydro-L-galactose (AHG), the main component of red macroalgae, was discovered in a marine bacterium, Vibrio sp. strain EJY3. However, the global metabolic network in response to AHG remains unclear. Here, the intracellular metabolites of EJY3 grown on AHG, glucose, or galactose were comparatively profiled using gas chromatography/time-of-flight mass spectrometry. The global metabolite profiling results revealed that the metabolic profile for AHG significantly differed from those for other common sugars. Specifically, the metabolic intermediate of the AHG pathway, 3,6-anhydrogalactonate, was detected during growth only in the presence of AHG; thus, the recently discovered key steps in AHG catabolism was found not to occur in the catabolism of other common sugars. Moreover, the levels of metabolic intermediates related to glycerolipid metabolism and valine biosynthesis were higher with AHG than those with other sugars. These comprehensive metabolomic analytical results for AHG in this marine bacterium can be used as the basis for having fermentative microbial strains to engineered to efficiently utilize AHG from macroalgal biomass.

Original languageEnglish
Pages (from-to)12-20
Number of pages9
JournalJournal of Biotechnology
Volume270
DOIs
Publication statusPublished - 2018 Mar 20

Fingerprint

Seaweed
Metabolomics
Galactose
Carbon
Bacteria
Metabolic Networks and Pathways
Carbohydrates
Vibrio
Metabolome
Valine
Gas Chromatography
Biomass
Mass Spectrometry

Keywords

  • 3,6-Anhydro-L-galactose
  • Metabolomics
  • Red macroalgae
  • Vibrio sp. strain EJY3

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Metabolomic response of a marine bacterium to 3,6-anhydro-L-galactose, the rare sugar from red macroalgae, as the sole carbon source. / Yun, Eun Ju; Yu, Sora; Kim, Sooah; Kim, Kyoung Heon.

In: Journal of Biotechnology, Vol. 270, 20.03.2018, p. 12-20.

Research output: Contribution to journalArticle

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