Metagenomic insight into methanogenic reactors promoting direct interspecies electron transfer via granular activated carbon

Jeong Hoon Park, Jong Hun Park, Hoon Je Seong, Woo Jun Sul, Kang Hyun Jin, Hee-Deung Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To provide insight into direct interspecies electron transfer via granular activated carbon (GAC), the effect of GAC supplementation on anaerobic digestion was evaluated. Compared to control samples, the GAC supplementation increased the total amount of methane production and its production rate by 31% and 72%, respectively. 16S rDNA sequencing analysis revealed a shift in the archaeal community composition; the Methanosarcina proportion decreased 17%, while the Methanosaeta proportion increased 5.6%. Metagenomic analyses based on shotgun sequencing demonstrated that the abundance of pilA and omcS genes belonging to Geobacter species decreased 69.4% and 29.4%, respectively. Furthermore, the analyses suggested a carbon dioxide reduction pathway rather than an acetate decarboxylation pathway for methane formation. Taken together, these results suggest that GAC improved methane production performance by shifting the microbial community and altering functional genes associated with direct interspecies electron transfer via conductive materials.

Original languageEnglish
Pages (from-to)414-422
Number of pages9
JournalBioresource Technology
Volume259
DOIs
Publication statusPublished - 2018 Jul 1

Fingerprint

Activated carbon
activated carbon
Methane
electron
Electrons
methane
Genes
Conductive materials
decarboxylation
Anaerobic digestion
gene
Ribosomal DNA
Carbon Dioxide
community composition
microbial community
acetate
Carbon dioxide
Acetates
carbon dioxide
reactor

Keywords

  • Anaerobic digestion
  • Direct interspecies electron transfer
  • Granular activated carbon
  • Metagenomics

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Metagenomic insight into methanogenic reactors promoting direct interspecies electron transfer via granular activated carbon. / Park, Jeong Hoon; Park, Jong Hun; Je Seong, Hoon; Sul, Woo Jun; Jin, Kang Hyun; Park, Hee-Deung.

In: Bioresource Technology, Vol. 259, 01.07.2018, p. 414-422.

Research output: Contribution to journalArticle

Park, Jeong Hoon ; Park, Jong Hun ; Je Seong, Hoon ; Sul, Woo Jun ; Jin, Kang Hyun ; Park, Hee-Deung. / Metagenomic insight into methanogenic reactors promoting direct interspecies electron transfer via granular activated carbon. In: Bioresource Technology. 2018 ; Vol. 259. pp. 414-422.
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