Methionine sulfoxide reductase B in the endoplasmic reticulum is critical for stress resistance and aging in Drosophila

Do Hwan Lim, Jee Yun Han, Jae Ryong Kim, Youngsik Lee, Hwa Young Kim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Methionine sulfoxide reductase B (MsrB) is an enzyme that repairs oxidatively damaged proteins by specifically reducing methionine-. R-sulfoxide back to methionine. Three MsrBs, localized in different cellular compartments, are expressed in mammals. However, the physiological roles of each MsrB with regard to its location remain poorly understood. Here, we expressed endoplasmic reticulum (ER)-targeted human MsrB3A (hMsrB3A) in Drosophila and examined its effects on various phenotypes. In two independent transgenic lines, both ubiquitous and neuronal expression of hMsrB3A rendered flies resistant to oxidative stress. Interestingly, these flies also showed significantly enhanced cold and heat tolerance. More strikingly, expression of hMsrB3A in the whole body and nervous system extended the lifespan of fruit flies at 29°C by 43-50% and 12-37%, respectively, suggesting that the targeted expression of MsrB in the ER regulates Drosophila lifespan. A significant increase in lifespan was also observed at 25°C only when hMsrB3A was expressed in neurons. Additionally, hMsrB3A overexpression significantly delayed the age-related decline in locomotor activity and fecundity. Taken together, our data provide evidence that the ER type of MsrB, MsrB3A, plays an important role in protection mechanisms against oxidative, cold and heat stresses and, moreover, in the regulation of fruit fly aging.

Original languageEnglish
Pages (from-to)20-26
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number1
DOIs
Publication statusPublished - 2012 Mar 2

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Methionine Sulfoxide Reductases
Endoplasmic Reticulum
Drosophila
Diptera
Aging of materials
sulfoxide
Fruits
Methionine
Fruit
Mammals
Oxidative stress
Neurology
Neurons
Locomotion
Repair
Nervous System
Fertility
Oxidative Stress
Hot Temperature
Phenotype

Keywords

  • ER
  • Fruit fly
  • Lifespan
  • Methionine sulfoxide reductase
  • MsrB
  • Stress tolerance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Methionine sulfoxide reductase B in the endoplasmic reticulum is critical for stress resistance and aging in Drosophila. / Lim, Do Hwan; Han, Jee Yun; Kim, Jae Ryong; Lee, Youngsik; Kim, Hwa Young.

In: Biochemical and Biophysical Research Communications, Vol. 419, No. 1, 02.03.2012, p. 20-26.

Research output: Contribution to journalArticle

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