Methods for the study of entotic cell death

Jens C. Hamann; Sung Eun Kim; Michael Overholtzer

doi:10.1007/978-1-4939-8873-0_28

Methods for the study of entotic cell death

Jens C. Hamann, Sung Eun Kim, Michael Overholtzer

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Citations (Scopus)

Abstract

Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	447-454
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-4939-8873-0_28
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1880
ISSN (Print)	1064-3745

Keywords

Anoikis
Cannibalism
Cell-in-cell
Engulfment
Entosis
Soft agar
Time-lapse

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-8873-0_28

Cite this

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title = "Methods for the study of entotic cell death",

abstract = "Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.",

keywords = "Anoikis, Cannibalism, Cell-in-cell, Engulfment, Entosis, Soft agar, Time-lapse",

author = "Hamann, {Jens C.} and Kim, {Sung Eun} and Michael Overholtzer",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",

year = "2019",

doi = "10.1007/978-1-4939-8873-0_28",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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TY - CHAP

T1 - Methods for the study of entotic cell death

AU - Hamann, Jens C.

AU - Kim, Sung Eun

AU - Overholtzer, Michael

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

AB - Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

KW - Anoikis

KW - Cannibalism

KW - Cell-in-cell

KW - Engulfment

KW - Entosis

KW - Soft agar

KW - Time-lapse

UR - http://www.scopus.com/inward/record.url?scp=85059496375&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8873-0_28

DO - 10.1007/978-1-4939-8873-0_28

M3 - Chapter

C2 - 30610714

AN - SCOPUS:85059496375

T3 - Methods in Molecular Biology

SP - 447

EP - 454

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Methods for the study of entotic cell death

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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