Methods for the study of entotic cell death

Jens C. Hamann, Sung Eun Kim, Michael Overholtzer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages447-454
Number of pages8
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1880
ISSN (Print)1064-3745

Keywords

  • Anoikis
  • Cannibalism
  • Cell-in-cell
  • Engulfment
  • Entosis
  • Soft agar
  • Time-lapse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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