Methyleugenol reduces cerebral ischemic injury by suppression of oxidative injury and inflammation

Yoo Keum Choi, Geum Sil Cho, Sunyoung Hwang, Byung Woo Kim, Ji H. Lim, Jae Chul Lee, Hyoung Chun Kim, Won-Ki Kim, Yeong Sik Kim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The present study tested the cytoprotective effect of methyleugenol in an in vivo ischemia model (i.e. middle cerebral artery occlusion (MCAO) for 1.5 h and subsequent reperfusion for 24 h) and further investigated its mechanism of action in in vitro cerebral ischemic models. When applied shortly after reperfusion, methyleugenol largely reduced cerebral ischemic injury. Methyleugenol decreased the caspase-3 activation and death of cultured cerebral cortical neurons caused by oxygen-glucose deprivation (OGD) for 1 h and subsequent re-oxygenation for 24 h. Methyleugenol markedly reduced superoxide generation in the ischemic brain and decreased the intracellular oxidative stress caused by OGD/re-oxygenation. It was found that methyleugenol elevated the activities of superoxide dismutase and catalase. Further, methyleugenol inhibited the production of nitric oxide and decreased the protein expression of inducible nitric oxide synthase. Methyleugenol down-regulated the production of pro-inflammatory cytokines in the ischemic brain as well as in immunostimulated mixed glial cells. The results indicate that methyleugenol could be useful for the treatment of ischemia/inflammation-related diseases.

Original languageEnglish
Pages (from-to)925-935
Number of pages11
JournalFree Radical Research
Volume44
Issue number8
DOIs
Publication statusPublished - 2010 Aug 1

Fingerprint

methyleugenol
Inflammation
Wounds and Injuries
Oxygenation
Reperfusion
Brain
Ischemia
Oxygen
Glucose
Oxidative stress
Middle Cerebral Artery Infarction
Nitric Oxide Synthase Type II

Keywords

  • ischemic injury
  • Methyleugenol
  • NO
  • OGD
  • ROS

ASJC Scopus subject areas

  • Biochemistry

Cite this

Choi, Y. K., Cho, G. S., Hwang, S., Kim, B. W., Lim, J. H., Lee, J. C., ... Kim, Y. S. (2010). Methyleugenol reduces cerebral ischemic injury by suppression of oxidative injury and inflammation. Free Radical Research, 44(8), 925-935. https://doi.org/10.3109/10715762.2010.490837

Methyleugenol reduces cerebral ischemic injury by suppression of oxidative injury and inflammation. / Choi, Yoo Keum; Cho, Geum Sil; Hwang, Sunyoung; Kim, Byung Woo; Lim, Ji H.; Lee, Jae Chul; Kim, Hyoung Chun; Kim, Won-Ki; Kim, Yeong Sik.

In: Free Radical Research, Vol. 44, No. 8, 01.08.2010, p. 925-935.

Research output: Contribution to journalArticle

Choi, Yoo Keum ; Cho, Geum Sil ; Hwang, Sunyoung ; Kim, Byung Woo ; Lim, Ji H. ; Lee, Jae Chul ; Kim, Hyoung Chun ; Kim, Won-Ki ; Kim, Yeong Sik. / Methyleugenol reduces cerebral ischemic injury by suppression of oxidative injury and inflammation. In: Free Radical Research. 2010 ; Vol. 44, No. 8. pp. 925-935.
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