Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Yoon Suk Kang, Juhyun Kim, Hyeon-Dong Shin, Young Do Nam, Jin Woo Bae, Che Ok Jeon, Woojun Park

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Abstract

A novel bacterial strain, designated PMB02T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2-1.5 mm in diameter after 3 days growth. The cells of strain PMB02T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20-30 °C, pH 6-8 and in media containing less than 1% NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 9 7.4 %, respectively. The values for DNA-DNA hybridization between strain PMB02T and M. aquaticum CCM 7218T and M. variabile GR3T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA-DNA relatedness data, strain PMB02T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02T (=KCTC 12901T=JCM 14648T).

Original languageEnglish
Pages (from-to)2849-2853
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume57
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

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ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

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