Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Yoon Suk Kang, Juhyun Kim, Hyeon-Dong Shin, Young Do Nam, Jin Woo Bae, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A novel bacterial strain, designated PMB02T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2-1.5 mm in diameter after 3 days growth. The cells of strain PMB02T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20-30 °C, pH 6-8 and in media containing less than 1% NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 9 7.4 %, respectively. The values for DNA-DNA hybridization between strain PMB02T and M. aquaticum CCM 7218T and M. variabile GR3T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA-DNA relatedness data, strain PMB02T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02T (=KCTC 12901T=JCM 14648T).

Original languageEnglish
Pages (from-to)2849-2853
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume57
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Platanus orientalis
Methylobacterium
DNA
new species
phylogenetics
leaves
rRNA Genes
gene
relatedness
phylogeny
agar
Genes
ribosomal RNA
Catalase
nucleotide sequences
Agar
Names
Oxidoreductases
quinones
nucleic acid hybridization

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis. / Kang, Yoon Suk; Kim, Juhyun; Shin, Hyeon-Dong; Nam, Young Do; Bae, Jin Woo; Jeon, Che Ok; Park, Woojun.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 57, No. 12, 01.12.2007, p. 2849-2853.

Research output: Contribution to journalArticle

Kang, Yoon Suk ; Kim, Juhyun ; Shin, Hyeon-Dong ; Nam, Young Do ; Bae, Jin Woo ; Jeon, Che Ok ; Park, Woojun. / Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis. In: International Journal of Systematic and Evolutionary Microbiology. 2007 ; Vol. 57, No. 12. pp. 2849-2853.
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