Micro corona discharge based cell lysis method suitable for inhibitor resistant bacterial sensing systems

Eun Hee Lee, Beelee Chua, Ahjeong Son

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We demonstrated a bacterial cell lysis method suitable for inhibitor resistant bacterial detection systems where purification of extracted DNA is not necessary. The presented method potentially improves the field portability of such systems. It allows cell lysis and DNA extraction to be performed without the use of bead mill, sonication, thermal cycling, additional reagents or enzymes. Bacterial cell lysis is achieved in a single step by pumping ozone generated by a micro corona discharge into the bacterial sample. The results with Pseudomonas putida as the target bacteria showed that it was capable of achieving 98.5 ± 0.2% lysis (normalized to 1 min of sonication at 10 W) after 10 min of treatment at a flow rate of 38 ml/min and an applied voltage of 2000 V. By increasing the treatment duration, flow rate and applied voltage, the normalized % lysis could be increased. In addition, continuous and pulsed treatments yield similar normalized % cell lysis.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume216
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Sonication
electric corona
inhibitors
DNA
Flow rate
Ozone
Electric potential
Thermal cycling
cells
Purification
Bacteria
Enzymes
deoxyribonucleic acid
flow velocity
pseudomonas
electric potential
purification
beads
bacteria
ozone

Keywords

  • Bacterial detection systems
  • Cell lysis
  • Corona discharge
  • Inhibitor resistant
  • Ozone

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

Micro corona discharge based cell lysis method suitable for inhibitor resistant bacterial sensing systems. / Lee, Eun Hee; Chua, Beelee; Son, Ahjeong.

In: Sensors and Actuators, B: Chemical, Vol. 216, 01.09.2015, p. 17-23.

Research output: Contribution to journalArticle

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