Abstract
We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1920-1922 |
| Number of pages | 3 |
| Volume | 3 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 Jan 1 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 2013 Oct 27 → 2013 Oct 31 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 13/10/27 → 13/10/31 |
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ASJC Scopus subject areas
- Bioengineering
Cite this
Microalgal culture, lipid production and extraction using an integrated microfluidic system. / Lim, H. S.; Kim, J. Y H; Kwak, H. S.; Sim, Sang Jun.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. p. 1920-1922.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Microalgal culture, lipid production and extraction using an integrated microfluidic system
AU - Lim, H. S.
AU - Kim, J. Y H
AU - Kwak, H. S.
AU - Sim, Sang Jun
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.
AB - We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.
KW - Culture
KW - Lipid extraction
KW - Lipid production
KW - Microalgae
KW - Microfluidics
KW - Micropillar filter
UR - http://www.scopus.com/inward/record.url?scp=84907321564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907321564&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907321564
SN - 9781632666246
VL - 3
SP - 1920
EP - 1922
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -