Microalgal culture, lipid production and extraction using an integrated microfluidic system

H. S. Lim, J. Y H Kim, H. S. Kwak, Sang Jun Sim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1920-1922
Number of pages3
Volume3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Microfluidics
Lipids
Thin layer chromatography
2-Propanol
Microchannels
Cell culture
Debris
Gas chromatography
Alcohols
Ethanol
Nitrogen

ASJC Scopus subject areas

  • Bioengineering

Cite this

Lim, H. S., Kim, J. Y. H., Kwak, H. S., & Sim, S. J. (2013). Microalgal culture, lipid production and extraction using an integrated microfluidic system. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 3, pp. 1920-1922). Chemical and Biological Microsystems Society.

Microalgal culture, lipid production and extraction using an integrated microfluidic system. / Lim, H. S.; Kim, J. Y H; Kwak, H. S.; Sim, Sang Jun.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. p. 1920-1922.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lim, HS, Kim, JYH, Kwak, HS & Sim, SJ 2013, Microalgal culture, lipid production and extraction using an integrated microfluidic system. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 3, Chemical and Biological Microsystems Society, pp. 1920-1922, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Lim HS, Kim JYH, Kwak HS, Sim SJ. Microalgal culture, lipid production and extraction using an integrated microfluidic system. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3. Chemical and Biological Microsystems Society. 2013. p. 1920-1922
Lim, H. S. ; Kim, J. Y H ; Kwak, H. S. ; Sim, Sang Jun. / Microalgal culture, lipid production and extraction using an integrated microfluidic system. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. pp. 1920-1922
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