Microalgal culture, lipid production and extraction using an integrated microfluidic system

H. S. Lim; J. Y H Kim; H. S. Kwak; Sang Jun Sim

Microalgal culture, lipid production and extraction using an integrated microfluidic system

H. S. Lim, J. Y H Kim, H. S. Kwak, Sang Jun Sim

Department of Chemical and Biological Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Publisher	Chemical and Biological Microsystems Society
Pages	1920-1922
Number of pages	3
Volume	3
ISBN (Print)	9781632666246
Publication status	Published - 2013 Jan 1
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 2013 Oct 27 → 2013 Oct 31

Other

Other	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country	Germany
City	Freiburg
Period	13/10/27 → 13/10/31

ASJC Scopus subject areas

Bioengineering

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Microalgal culture, lipid production and extraction using an integrated microfluidic system. / Lim, H. S.; Kim, J. Y H; Kwak, H. S.; Sim, Sang Jun.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. p. 1920-1922.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lim, HS, Kim, JYH, Kwak, HS & Sim, SJ 2013, Microalgal culture, lipid production and extraction using an integrated microfluidic system. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 3, Chemical and Biological Microsystems Society, pp. 1920-1922, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.

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abstract = "We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.",

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AB - We present a novel method for simple and efficient lipid analysis by integrating multi-process including microalgal culture, lipid production and lipid extraction on a single microfluidic device. We devised a double-layered PDMS device, composed of cell culture chamber and connecting channel filled with micropillar array between culture chamber and output reservoir. Lipid accumulation was induced by supply of nitrogen deficient media into microchannel and lipid extraction was performed by alcohol solvents such as 70% ethanol, isopropanol. Cell debris was removed by micropillar filter and lipid extract from output reservoir was analyzed by thin layer chromatography and gas chromatography.

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KW - Lipid extraction

KW - Lipid production

KW - Microalgae

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KW - Micropillar filter

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