Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer: Identification of candidate genes in pathogenesis of invasion in cervical cancer

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Abstract

The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.

Original languageEnglish
Pages (from-to)1051-1059
Number of pages9
JournalInternational Journal of Gynecological Cancer
Volume18
Issue number5
DOIs
Publication statusPublished - 2008 Sep 1

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Carcinoma in Situ
Genetic Association Studies
Microarray Analysis
Cervix Uteri
Uterine Cervical Neoplasms
Genes
Down-Regulation
Reverse Transcription
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Transcriptome
Up-Regulation
Technology

Keywords

  • Candidate genes
  • Cervical cancer
  • CIS
  • Gene expression
  • Microarray

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

Cite this

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title = "Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer: Identification of candidate genes in pathogenesis of invasion in cervical cancer",
abstract = "The objective of this study was to identify genes that are related to pathogenesis of carcinoma in situ (CIS) to invasive cervical cancer with the use of oligonucleotide microarray and reverse transcription-polymerase chain reaction (RT-PCR). Each two cases of normal cervix, CIS, and invasive cervical cancer were investigated with DNA microarray technology. Differential gene expression profiles among them were analyzed. Expression levels of selected genes from the microarray results were confirmed by RT-PCR. The expressions of 15,286 genes were compared and 458 genes were upregulated or downregulated by twofold or more compared with each other group. Among 458 genes, 22 genes were upregulated and 40 genes were downregulated by twofold or more in invasive cervical cancer group compared with CIS group. RT-PCR analysis confirmed upregulation of 18 genes and downregulation of 5 genes in invasive cervical cancer group. RBP1, TFRC, SPP1, SAA1, ARHGAP8, and NDRG1, which were upregulated, and GATA3, PLAGL1, APOD, DUSP1, and CYR61, which were downregulated, were considered as candidate genes associated with invasion of cervical cancer.",
keywords = "Candidate genes, Cervical cancer, CIS, Gene expression, Microarray",
author = "Song, {Jae Yun} and Lee, {Jae Kwan} and Lee, {Nak Woo} and Jung, {Hak Hyun} and Kim, {S. H.} and Lee, {K. W.}",
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