Microarray profiling of gene expression in aging and its alteration by caloric restriction in mice

Richard Weindruch, Tsuyoshi Kayo, Cheol Koo Lee, Tomas A. Prolla

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

An active research area in biological gerontology concerns the mechanisms by which caloric restriction (CR) retards the aging process in laboratory rodents. We used high density oligonucleotide arrays representing 6347 genes to determine the gene expression profile of the aging process in gastrocnemius muscle of male C57BL/6 mice. Aging resulted in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes. Most alterations were completely or partially prevented by CR. Transcriptional patterns of muscle from calorie-restricted animals suggest that CR retards the aging process by causing a metabolic shift toward increased protein turnover and decreased macromolecular damage. The use of high density oligonucleotide microarrays provides a new tool to measure biological age on a tissue-specific basis and to evaluate at the molecular level the efficacy of nutritional interventions designed to retard the aging process.

Original languageEnglish
Pages (from-to)918S-923S
JournalJournal of Nutrition
Volume131
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Aging
  • Caloric restriction
  • Gene expression
  • Muscle
  • Oligonucleotide microarrays

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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