Microbial cell arrays

Tal Elad, Jin Hyung Lee, Man Bock Gu, Shimshon Belkin

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Citations (Scopus)

Abstract

The coming of age of whole-cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines - the whole cell array. In the present chapter, we highlight the state-of-the-art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals, and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of highperformance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis, and - most importantly - enhanced long term maintenance of viability and activity on the fabricated biochips.

Original languageEnglish
Title of host publicationAdvances in Biochemical Engineering/Biotechnology
EditorsT. Scheper
Pages85-108
Number of pages24
DOIs
Publication statusPublished - 2010 Aug 19

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
Volume117
ISSN (Print)0724-6145

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Keywords

  • Bioreporters
  • Biosensors
  • Cell deposition
  • Cell immobilization
  • Gene expression
  • Toxicity testing
  • Whole-cell arrays

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Elad, T., Lee, J. H., Gu, M. B., & Belkin, S. (2010). Microbial cell arrays. In T. Scheper (Ed.), Advances in Biochemical Engineering/Biotechnology (pp. 85-108). (Advances in Biochemical Engineering/Biotechnology; Vol. 117). https://doi.org/10.1007/10_2009_16