TY - JOUR
T1 - Microbial pathway engineering for industrial processes
T2 - Evolution, combinatorial biosynthesis and rational design
AU - Rohlin, Lars
AU - Oh, Min Kyu
AU - Liao, James C.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Microbial pathway engineering has made significant progress in multiple areas. Many examples of successful pathway engineering for specialty and fine chemicals have been reported in the past two years. Novel carotenoids and polyketides have been synthesized using molecular evolution and combinatorial strategies. In addition, rational design approaches based on metabolic control have been reported to increase metabolic flux to specific products. Experimental and computational tools have been developed to aid in design, reconstruction and analysis of non-native pathways. It is expected that a hybrid of evolutionary, combinatorial and rational design approaches will yield significant advances in the near future.
AB - Microbial pathway engineering has made significant progress in multiple areas. Many examples of successful pathway engineering for specialty and fine chemicals have been reported in the past two years. Novel carotenoids and polyketides have been synthesized using molecular evolution and combinatorial strategies. In addition, rational design approaches based on metabolic control have been reported to increase metabolic flux to specific products. Experimental and computational tools have been developed to aid in design, reconstruction and analysis of non-native pathways. It is expected that a hybrid of evolutionary, combinatorial and rational design approaches will yield significant advances in the near future.
UR - http://www.scopus.com/inward/record.url?scp=0034986950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034986950&partnerID=8YFLogxK
U2 - 10.1016/S1369-5274(00)00213-7
DO - 10.1016/S1369-5274(00)00213-7
M3 - Review article
C2 - 11378488
AN - SCOPUS:0034986950
VL - 4
SP - 330
EP - 335
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
SN - 1369-5274
IS - 3
ER -