Microbial pathway engineering for industrial processes: Evolution, combinatorial biosynthesis and rational design

Lars Rohlin, Min-Kyu Oh, James C. Liao

Research output: Contribution to journalArticle

43 Citations (Scopus)


Microbial pathway engineering has made significant progress in multiple areas. Many examples of successful pathway engineering for specialty and fine chemicals have been reported in the past two years. Novel carotenoids and polyketides have been synthesized using molecular evolution and combinatorial strategies. In addition, rational design approaches based on metabolic control have been reported to increase metabolic flux to specific products. Experimental and computational tools have been developed to aid in design, reconstruction and analysis of non-native pathways. It is expected that a hybrid of evolutionary, combinatorial and rational design approaches will yield significant advances in the near future.

Original languageEnglish
Pages (from-to)330-335
Number of pages6
JournalCurrent Opinion in Microbiology
Issue number3
Publication statusPublished - 2001 Jun 15
Externally publishedYes


ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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