Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli

Suman Mazumdar, Jinwon Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A variety of biofuel and biorefinery products have been produced from engineered Escherichia coli till date. Most of these products had been derived from simple sugars in its pure form, rather than deriving it from alternative, renewable and carbon neutral sources, such as marine alga biomass. Engineering E. coli to use algal hydrolysate can make these an attractive carbon source for the industrial production of value added fuels and chemicals. This work reports the engineering of E. coli by a combination of gene deletion and synthetic pathway incorporation, for the efficient utilization of algal hydrolysate to produce BA (2,3 butanediol. +. acetoin) under microaerobic condition. Engineered strain produced ~19. g/L of total BA from algal hydrolysate in defined M9 salt media at a yield of 0.43. g/g.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalBioresource Technology
Volume136
DOIs
Publication statusPublished - 2013 May 1

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Seaweed
seaweed
Acetoin
Escherichia coli
engineering
industrial production
carbon
Carbon
biofuel
sugar
Biofuels
alga
Algae
salt
Sugars
gene
biomass
Biomass
Salts
Genes

Keywords

  • 2,3 Butanediol
  • Escherichia coli
  • Mannitol
  • Metabolic engineering
  • Seaweeds

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli. / Mazumdar, Suman; Lee, Jinwon; Oh, Min-Kyu.

In: Bioresource Technology, Vol. 136, 01.05.2013, p. 329-336.

Research output: Contribution to journalArticle

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