Microbial production of ethanol from acetate by engineered Ralstonia eutropha

Hye Mi Lee, Bo Young Jeon, Min-Kyu Oh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study was performed to produce ethanol from acetate using a genetically engineered Ralstonia eutropha. In order to genetically modify R. eutropha H16, phaCAB operon encoding metabolic pathway genes from acetyl-CoA to polyhydroxybutyrate (PHB) was deleted and adhE encoding an alcohol dehydrogenase from Escherichia coli was overexpressed for conversion of acetyl-CoA to ethanol. The resulting strain produced ethanol up to 170 mg/L when cultivated in minimal media supplemented with 5 g/L of acetate as a sole carbon source. Growth and ethanol production were optimized by adjusting nitrogen source (NH4Cl) content and repetitive feeding of acetate into the bacterial culture, by which the ethanol production was reached to approximately 350 mg/L for 84 h.

Original languageEnglish
Pages (from-to)402-407
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume21
Issue number3
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • acetate
  • ethanol
  • metabolic engineering
  • Ralstonia eutropha

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

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