Microcarrier culture of bowes melanoma cells in serum‐free medium with Human plasma fraction IV‐4+ V

Eui‐Cheol ‐C Jo, Man Bock Gu, Dong‐II ‐I Kim, Sung Bok Paik

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bowes melanoma cells were cultivated successfully in a serum‐free medium which was constructed by the concept of maximum retention of proteins from fractionated human plasma having growth stimulatory activities. The cells could be cultivated in the serum‐free medium without any adaptation period. The major serum‐free component of the medium was the fraction IV‐4 + V of the Cohn fractionation process of human plasma. Approximately six times increase of tissue‐type plasminogen activator (t‐PA) activity as compared with that in serum‐free medium even though the cell growth was much slower. In addition, the growth stimulatory activities of thrombin and fibronectin were investigated during the cultivation of Bowes melanoma cells in this serum‐free medium. These proteins contributed significantly to the enhanced growth of cells by reducing doubling time to 25 and 35 h as compared with 55 h in the serum‐free medium without them. Especially, fibronectin supported cells to propagate near to the maximum cell density achieved in the medium with 10% FBS.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalBiotechnology and Bioengineering
Volume38
Issue number3
DOIs
Publication statusPublished - 1991 Jul
Externally publishedYes

Keywords

  • fractionated human plasma
  • growth stimulatory activities
  • plasminogen activator
  • serum‐free medium

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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