Microfabricated fluorescence-activated cell sorter with hydrodynamic flow manipulation

Hyunwoo Bang, Chanil Chung, Jung Kyung Kim, Seonghwan Kim, Seok Chung, Dong Chul Han, Jun Keun Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Presented is a novel flow manipulation and particle detection method for the microfabricated fluorescence-activated cell sorter (μFACS). With hydrodynamic flow manipulation which includes passive flow channeling and hydrodynamic actuation with nozzle flow, we have developed a fast and robust method for utilizing the sorting function. These techniques are directly related to enabling the high throughput screening of the FACS machine. Moreover by detecting with synchronized imaging, we have also developed an on-line calibration technique for the accurate timing between detection and actuation. And this means that our system is very flexible that it can be tuned to any assay conditions with various flow speeds, particle densities and buffer or sample viscosities by real time calibration.

Original languageEnglish
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages50-54
Number of pages5
Volume1
Publication statusPublished - 2004 Nov 2
Externally publishedYes
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: 2004 Mar 72004 Mar 11

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period04/3/704/3/11

Keywords

  • Hydrodynamic flow manipulation
  • Lab-on-a-chip
  • Micro-FACS
  • PDMS
  • Plastic micromachining

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Bang, H., Chung, C., Kim, J. K., Kim, S., Chung, S., Han, D. C., & Chang, J. K. (2004). Microfabricated fluorescence-activated cell sorter with hydrodynamic flow manipulation. In M. Laudon, & B. Romanowicz (Eds.), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 (Vol. 1, pp. 50-54)