TY - GEN
T1 - MICROFLUIDIC CELL SHEARING ENABLES HIGHLY EFFECTIVE MACROMOLECULE INTRACELLULAR DELIVERY
AU - Kwon, Chan
AU - Kang, Geoum Young
AU - Chung, Aram
N1 - Funding Information:
This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFCIT1802-03) and the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2021R1A2C2006224).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.
AB - A novel microfluidic cell stretching platform using viscoelastic fluids is presented, enabling highly effective intracellular delivery of external biomolecules into various cell types. The cell suspended in methylcellulose (MC) solution is injected into the platform, where cells rapidly pass through a single constriction with a dimension slightly smaller than the cell diameter. Because of the MC solution, a high shear force is applied to the cells, effectively generating transient cell membrane discontinuities. With this approach, a high delivery efficiency (>97%), high throughput (>106 cells/min) and nearly clogging-free of various macromolecules into different immune cells were demonstrated for cancer immunotherapy applications.
KW - Cancer immunotherapy
KW - Intracellular delivery
KW - Microfluidic cell shearing
KW - mRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=85136921074&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136921074
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 649
EP - 650
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -
