Microfluidic chip-based fabrication of PLGA microfiber scaffolds for tissue engineering

Chang Mo Hwang, Ali Khademhosseini, Yongdoo Park, Kyung Sun, Sang Hoon Lee

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

In this paper, we have developed a method to produce poly(lactic-co- glycolic acid) (PLGA) microfibers within a microfluidic chip for the generation of 3D tissue engineering scaffolds. The synthesis of PLGA fibers was achieved by using a polydimethylsiloxane (PDMS)-based microfluidic spinning device in which linear streams of PLGA dissolved in dimethyl sulfoxide (DMSO) were precipitated in a glycerol-containing water solution. By changing the flow rate of PLGA solution from 1 to 50 μL/min with a sheath flow rate of 250 or 1000 μL/min, fibers were formed with diameters that ranged from 20 to 230 μm. The PLGA fibers were comprised of a dense outer surface and a highly porous interior. To evaluate the applicability of PLGA microfibers generated in this process as a cell culture scaffold, L929 fibroblasts were seeded on the PLGA fibers either as-fabricated or coated with fibronectin. L929 fibroblasts showed no significant difference in proliferation on both PLGA microfibers after 5 days of culture. As a test for application as nerve guide, neural progenitor cells were cultured and the neural axons elongated along the PLGA microfibers. Thus our experiments suggest that microfluidic chip-based PLGA microfiber fabrication may be useful for 3D cell culture tissue engineering applications.

Original languageEnglish
Pages (from-to)6845-6851
Number of pages7
JournalLangmuir
Volume24
Issue number13
DOIs
Publication statusPublished - 2008 Jul 1

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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