Microfluidic electrode array chip for electrical stimulation-mediated axonal regeneration

Ji Woon Kim, Yoon Young Choi, Si Hyung Park, Jang Ho Ha, Hee Uk Lee, Taewook Kang, Woong Sun, Bong Geun Chung

Research output: Contribution to journalArticlepeer-review

Abstract

The precise manipulation of the neural stem cell (NSC)-derived neural differentiation is still challenging, and there is a technological barrier to regulate the axonal regeneration in a controlled manner. Here, we developed a microfluidic chip integrated with a microelectrode array as an axonal guidance platform. The microfluidic electrode array chip consisted of two compartments and a bridge microchannel that could isolate and guide the axons. We demonstrated that the NSCs were largely differentiated into neural cells as the electric field was applied to the microfluidic electrode array chip. We also confirmed the synergistic effects of the electrical stimulation (ES) and neurotrophic factor (NF) on axonal outgrowth. This microfluidic electrode array chip can serve as a central nervous system (CNS) model for axonal injury and regeneration. Therefore, it could be a potentially powerful tool for an in vitro model of the axonal regeneration.

Original languageEnglish
JournalLab on a Chip
DOIs
Publication statusAccepted/In press - 2022

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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