Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis

Jaoon Young Hwan Kim, Ho Seok Kwak, Young Joon Sung, Hong Il Choi, Min Eui Hong, Hyun Seok Lim, Jae Hyeok Lee, Sang Yup Lee, Sang Jun Sim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Microalgae possess great potential as a source of sustainable energy, but the intrinsic inefficiency of photosynthesis is a major challenge to realize this potential. Photosynthetic organisms evolved phototaxis to find optimal light condition for photosynthesis. Here we report a microfluidic screening using competitive phototaxis of the model alga, Chlamydomonas reinhardtii, for rapid isolation of strains with improved photosynthetic efficiencies. We demonstrated strong relationship between phototaxis and photosynthetic efficiency by quantitative analysis of phototactic response at the single-cell level using a microfluidic system. Based on this positive relationship, we enriched the strains with improved photosynthetic efficiency by isolating cells showing fast phototactic responses from a mixture of 10,000 mutants, thereby greatly improving selection efficiency over 8 fold. Among 147 strains isolated after screening, 94.6% showed improved photoautotrophic growth over the parental strain. Two mutants showed much improved performances with up to 1.9- and 8.1-fold increases in photoautotrophic cell growth and lipid production, respectively, a substantial improvement over previous approaches. We identified candidate genes that might be responsible for fast phototactic response and improved photosynthesis, which can be useful target for further strain engineering. Our approach provides a powerful screening tool for rapid improvement of microalgal strains to enhance photosynthetic productivity.

Original languageEnglish
Article number21155
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Feb 8

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Microfluidics
Photosynthesis
Renewable Energy
Microalgae
Chlamydomonas reinhardtii
Growth
Lipids
Light
Genes
Phototaxis

ASJC Scopus subject areas

  • General

Cite this

Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis. / Kim, Jaoon Young Hwan; Kwak, Ho Seok; Sung, Young Joon; Choi, Hong Il; Hong, Min Eui; Lim, Hyun Seok; Lee, Jae Hyeok; Lee, Sang Yup; Sim, Sang Jun.

In: Scientific Reports, Vol. 6, 21155, 08.02.2016.

Research output: Contribution to journalArticle

Kim, Jaoon Young Hwan ; Kwak, Ho Seok ; Sung, Young Joon ; Choi, Hong Il ; Hong, Min Eui ; Lim, Hyun Seok ; Lee, Jae Hyeok ; Lee, Sang Yup ; Sim, Sang Jun. / Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis. In: Scientific Reports. 2016 ; Vol. 6.
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