Microfluidic immunoassay for point-of-care testing using simple fluid vent control

Jaehoon Kim, Kihun Hong, Hyunho Kim, Joonseok Seo, Jaeun Jeong, Pan Kee Bae, Yong Beom Shin, Jeong Hoon Lee, Hyun Jeong Oh, Seok Chung

Research output: Contribution to journalArticle

Abstract

Microfluidic immunoassays have gained immense importance as a point-of-care testing (POCT) platform owing to their low reagent volume requirements, low cost, portability, and rapid reaction. However, they are characterized by inconsistency and lack of reproducibility. In this study, we developed a microfluidic immunoassay platform with a simple signal enhancement technique using fluid vent control. The components of this fluorescence-based immunoassay were successfully pre-loaded in the microfluidic device. The analytes reacted with the detection antibody conjugated on fluorescence beads, and bound to capture the antibody immobilized zone during channel flow. The fluid vent was closed using a small PC-connected equipment when the fluid reached the target displacement. The pausing of the fluid provided sufficient time for the immune reaction, and this addition of the fluid pausing significantly enhanced the immune reaction and increased the limit of detection (LOD) of the immunoassay. The LOD of the developed assay was the same as that of the sandwich fluorescence immunoassay in terms of quantity. Furthermore, the LOD was significantly higher than that of the commercial rapid kits. Therefore, our simple technique could be employed for cost-effective and accurate immunoassays in the field of POCT.

Original languageEnglish
Article number128094
JournalSensors and Actuators, B: Chemical
Volume316
DOIs
Publication statusPublished - 2020 Aug 1

Keywords

  • immunoassay
  • microfluidics
  • point-of-care-testing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Kim, J., Hong, K., Kim, H., Seo, J., Jeong, J., Bae, P. K., Shin, Y. B., Lee, J. H., Oh, H. J., & Chung, S. (2020). Microfluidic immunoassay for point-of-care testing using simple fluid vent control. Sensors and Actuators, B: Chemical, 316, [128094]. https://doi.org/10.1016/j.snb.2020.128094