Microfluidic in-reservoir pre-concentration using a buffer drain technique

Junghyo Yoon, Youngkyu Cho, Sewoon Han, Chae Seung Lim, Jeong Hoon Lee, Seok Chung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 μl of buffer was successfully drained from a 100 μl sample, resulting in a 4-fold increase in influenza hemagglutinin concentration in the reservoir. The volume of the final concentrated sample was suitable for detection of influenza hemagglutinin by the enzyme-linked immunosorbent assay, demonstrating the usefulness of the developed platform for enhanced sensitivity of virus detection in a conventional analysis. This journal is

Original languageEnglish
Pages (from-to)2778-2782
Number of pages5
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume14
Issue number15
DOIs
Publication statusPublished - 2014 Aug 7

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Microfluidics
Viruses
Buffers
Hemagglutinins
Lab-On-A-Chip Devices
Human Influenza
Ions
Orthomyxoviridae
Immunosorbents
Enzyme-Linked Immunosorbent Assay
Assays
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Microfluidic in-reservoir pre-concentration using a buffer drain technique. / Yoon, Junghyo; Cho, Youngkyu; Han, Sewoon; Lim, Chae Seung; Lee, Jeong Hoon; Chung, Seok.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 15, 07.08.2014, p. 2778-2782.

Research output: Contribution to journalArticle

Yoon, Junghyo ; Cho, Youngkyu ; Han, Sewoon ; Lim, Chae Seung ; Lee, Jeong Hoon ; Chung, Seok. / Microfluidic in-reservoir pre-concentration using a buffer drain technique. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2014 ; Vol. 14, No. 15. pp. 2778-2782.
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