Microfluidic intracellular delivery via fluid cell shearing

Geoum Young Kang, Chan Kwon, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a novel microfluidic vector-less intracellular delivery platform that effectively internalizes various nanomaterials into different immune cell lines without the use of external forces. The cells suspended in Methylcellulose solution are designed to pass a single constriction where high shear stresses and pressure gradients are imposed to deform cells. Due to cell shearing, transient nanopores on cell membranes are effectively generated, and target nanomaterials from the surrounding medium can be easily diffused into the cell cytosol. Using this approach, highly efficient, high-throughput, and rapid delivery of macromolecules into different cell lines was accomplished while maintaining high cell viability.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1033-1034
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 2020 Oct 42020 Oct 9

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

Keywords

  • Cell Stretching
  • Fluid Cell Shearing
  • Intracellular Delivery
  • Nanomaterial Delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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