Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Sang Min Paik, Sang Jun Sim, Noo Li Jeon

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4 +/PO4 2 , C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalBioresource Technology
Publication statusPublished - 2017


  • Continuous flow
  • Microalgae
  • Microfluidic perfusion bioreactor
  • Nutrient gradient
  • Triacylglycerol

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal


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