Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Sang Min Paik, Sang Jun Sim, Noo Li Jeon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4 +/PO4 2 , C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

Original languageEnglish
Pages (from-to)433-437
Number of pages5
JournalBioresource Technology
Volume233
DOIs
Publication statusPublished - 2017

Fingerprint

Bioreactors
Microfluidics
bioreactor
Lipids
Nutrients
Productivity
lipid
secondary metabolite
Metabolites
productivity
nutrient
Biomass
fold
biomass

Keywords

  • Continuous flow
  • Microalgae
  • Microfluidic perfusion bioreactor
  • Nutrient gradient
  • Triacylglycerol

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity. / Paik, Sang Min; Sim, Sang Jun; Jeon, Noo Li.

In: Bioresource Technology, Vol. 233, 2017, p. 433-437.

Research output: Contribution to journalArticle

@article{0e0084a058c348c5b1d10f537453aa6e,
title = "Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity",
abstract = "Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5{\%}/7.5{\%} of NH4 +/PO4 2 −, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.",
keywords = "Continuous flow, Microalgae, Microfluidic perfusion bioreactor, Nutrient gradient, Triacylglycerol",
author = "Paik, {Sang Min} and Sim, {Sang Jun} and Jeon, {Noo Li}",
year = "2017",
doi = "10.1016/j.biortech.2017.02.050",
language = "English",
volume = "233",
pages = "433--437",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

AU - Paik, Sang Min

AU - Sim, Sang Jun

AU - Jeon, Noo Li

PY - 2017

Y1 - 2017

N2 - Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4 +/PO4 2 −, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

AB - Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4 +/PO4 2 −, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4 + depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

KW - Continuous flow

KW - Microalgae

KW - Microfluidic perfusion bioreactor

KW - Nutrient gradient

KW - Triacylglycerol

UR - http://www.scopus.com/inward/record.url?scp=85014579428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014579428&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2017.02.050

DO - 10.1016/j.biortech.2017.02.050

M3 - Article

C2 - 28279610

AN - SCOPUS:85014579428

VL - 233

SP - 433

EP - 437

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -