Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

Seok Chung; R. Sudo; I. Zervantonakis; T. Rimchala; P. J. Mack; C. R. Wan; V. Vickerman; R. D. Kamm

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

Seok Chung, R. Sudo, I. Zervantonakis, T. Rimchala, P. J. Mack, C. R. Wan, V. Vickerman, R. D. Kamm

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

Original language	English
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
Publisher	Chemical and Biological Microsystems Society
Pages	24-26
Number of pages	3
Publication status	Published - 2008 Jan 1
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Chung, S., Sudo, R., Zervantonakis, I., Rimchala, T., Mack, P. J., Wan, C. R., Vickerman, V., & Kamm, R. D. (2008). Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 24-26). Chemical and Biological Microsystems Society.

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. / Chung, Seok; Sudo, R.; Zervantonakis, I. et al.

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 24-26.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chung, S, Sudo, R, Zervantonakis, I, Rimchala, T, Mack, PJ, Wan, CR, Vickerman, V & Kamm, RD 2008, Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 24-26, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08/10/12.

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abstract = "Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.",

keywords = "Capillary morphogenesis, Cell migration, Microfluidic",

author = "Seok Chung and R. Sudo and I. Zervantonakis and T. Rimchala and Mack, {P. J.} and Wan, {C. R.} and V. Vickerman and Kamm, {R. D.}",

year = "2008",

month = jan,

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booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",

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AU - Chung, Seok

AU - Sudo, R.

AU - Zervantonakis, I.

AU - Rimchala, T.

AU - Mack, P. J.

AU - Wan, C. R.

AU - Vickerman, V.

AU - Kamm, R. D.

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N2 - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

AB - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

KW - Capillary morphogenesis

KW - Cell migration

KW - Microfluidic

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

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ASJC Scopus subject areas

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