Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

Seok Chung, R. Sudo, I. Zervantonakis, T. Rimchala, P. J. Mack, C. R. Wan, V. Vickerman, R. D. Kamm

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages24-26
Number of pages3
Publication statusPublished - 2008 Jan 1
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Chung, S., Sudo, R., Zervantonakis, I., Rimchala, T., Mack, P. J., Wan, C. R., Vickerman, V., & Kamm, R. D. (2008). Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 24-26). Chemical and Biological Microsystems Society.