Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber

Jae Hoon Jung, Chang Hyung Choi, Seok Chung, Young Min Chung, Chang Soo Lee

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We present a simple synthetic approach for the preparation of cell attachable Janus polyurethane (PU) microfibers in a microfluidic system. The synthesis was performed by using laminar flows of multiple streams with spontaneous formation of carbon dioxide bubbles resulting in an asymmetrically porous PU microfiber. The fabricated asymmetric microfiber (Janus microfiber) provides two distinctive properties: one is a porous region to promote the cellular adhesion and the other is a nonporous region rendering the mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. Cells cultured on the fibers easily bridged gaps between microfibers by joining together to form a cell sheet. The maximum distance between fibers that fibroblasts bridged is approximately 200 m over 15 days. The Janus microfiber can be used for not only an alternative 2D cell culture plate but also as a novel 3D scaffold for tissue engineering without any need for elegant surface modification for enhancing cell adhesions.

Original languageEnglish
Pages (from-to)2596-2602
Number of pages7
JournalLab on a Chip
Volume9
Issue number17
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber'. Together they form a unique fingerprint.

  • Cite this