Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber

Jae Hoon Jung, Chang Hyung Choi, Seok Chung, Young Min Chung, Chang Soo Lee

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We present a simple synthetic approach for the preparation of cell attachable Janus polyurethane (PU) microfibers in a microfluidic system. The synthesis was performed by using laminar flows of multiple streams with spontaneous formation of carbon dioxide bubbles resulting in an asymmetrically porous PU microfiber. The fabricated asymmetric microfiber (Janus microfiber) provides two distinctive properties: one is a porous region to promote the cellular adhesion and the other is a nonporous region rendering the mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. Cells cultured on the fibers easily bridged gaps between microfibers by joining together to form a cell sheet. The maximum distance between fibers that fibroblasts bridged is approximately 200 m over 15 days. The Janus microfiber can be used for not only an alternative 2D cell culture plate but also as a novel 3D scaffold for tissue engineering without any need for elegant surface modification for enhancing cell adhesions.

Original languageEnglish
Pages (from-to)2596-2602
Number of pages7
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number17
DOIs
Publication statusPublished - 2009 Sep 28
Externally publishedYes

Fingerprint

Polyurethanes
Microfluidics
Cell adhesion
Cell culture
Cell Adhesion
Adhesives
Fibers
Fibroblasts
Tissue Engineering
Scaffolds (biology)
Laminar flow
Tissue engineering
Carbon Dioxide
Scaffolds
Joining
Strength of materials
Surface treatment
Cultured Cells
Cell Survival
Carbon dioxide

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber. / Jung, Jae Hoon; Choi, Chang Hyung; Chung, Seok; Chung, Young Min; Lee, Chang Soo.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 17, 28.09.2009, p. 2596-2602.

Research output: Contribution to journalArticle

Jung, Jae Hoon ; Choi, Chang Hyung ; Chung, Seok ; Chung, Young Min ; Lee, Chang Soo. / Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 17. pp. 2596-2602.
@article{602ca0282c7045c7bdb17686946ae309,
title = "Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber",
abstract = "We present a simple synthetic approach for the preparation of cell attachable Janus polyurethane (PU) microfibers in a microfluidic system. The synthesis was performed by using laminar flows of multiple streams with spontaneous formation of carbon dioxide bubbles resulting in an asymmetrically porous PU microfiber. The fabricated asymmetric microfiber (Janus microfiber) provides two distinctive properties: one is a porous region to promote the cellular adhesion and the other is a nonporous region rendering the mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. Cells cultured on the fibers easily bridged gaps between microfibers by joining together to form a cell sheet. The maximum distance between fibers that fibroblasts bridged is approximately 200 m over 15 days. The Janus microfiber can be used for not only an alternative 2D cell culture plate but also as a novel 3D scaffold for tissue engineering without any need for elegant surface modification for enhancing cell adhesions.",
author = "Jung, {Jae Hoon} and Choi, {Chang Hyung} and Seok Chung and Chung, {Young Min} and Lee, {Chang Soo}",
year = "2009",
month = "9",
day = "28",
doi = "10.1039/b901308c",
language = "English",
volume = "9",
pages = "2596--2602",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "17",

}

TY - JOUR

T1 - Microfluidic synthesis of a cell adhesive Janus polyurethane microfiber

AU - Jung, Jae Hoon

AU - Choi, Chang Hyung

AU - Chung, Seok

AU - Chung, Young Min

AU - Lee, Chang Soo

PY - 2009/9/28

Y1 - 2009/9/28

N2 - We present a simple synthetic approach for the preparation of cell attachable Janus polyurethane (PU) microfibers in a microfluidic system. The synthesis was performed by using laminar flows of multiple streams with spontaneous formation of carbon dioxide bubbles resulting in an asymmetrically porous PU microfiber. The fabricated asymmetric microfiber (Janus microfiber) provides two distinctive properties: one is a porous region to promote the cellular adhesion and the other is a nonporous region rendering the mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. Cells cultured on the fibers easily bridged gaps between microfibers by joining together to form a cell sheet. The maximum distance between fibers that fibroblasts bridged is approximately 200 m over 15 days. The Janus microfiber can be used for not only an alternative 2D cell culture plate but also as a novel 3D scaffold for tissue engineering without any need for elegant surface modification for enhancing cell adhesions.

AB - We present a simple synthetic approach for the preparation of cell attachable Janus polyurethane (PU) microfibers in a microfluidic system. The synthesis was performed by using laminar flows of multiple streams with spontaneous formation of carbon dioxide bubbles resulting in an asymmetrically porous PU microfiber. The fabricated asymmetric microfiber (Janus microfiber) provides two distinctive properties: one is a porous region to promote the cellular adhesion and the other is a nonporous region rendering the mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. Cells cultured on the fibers easily bridged gaps between microfibers by joining together to form a cell sheet. The maximum distance between fibers that fibroblasts bridged is approximately 200 m over 15 days. The Janus microfiber can be used for not only an alternative 2D cell culture plate but also as a novel 3D scaffold for tissue engineering without any need for elegant surface modification for enhancing cell adhesions.

UR - http://www.scopus.com/inward/record.url?scp=70349337713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349337713&partnerID=8YFLogxK

U2 - 10.1039/b901308c

DO - 10.1039/b901308c

M3 - Article

VL - 9

SP - 2596

EP - 2602

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 17

ER -